Effect Of AMF On Plant Growth And Substrate Characteristics Of Vegetation Concrete

Vegetation concrete ecological restoration technology,witch has the dual function of engineering protection and vegetation restoration,is one of a Slope Ecological Restoration Technology.Vegetation concrete is able to create stable growth environment for slope vegetation by attach the concrete substrate to the rock surface with the help of wire mesh,anchor nails and concrete arranged on slope,shows a vast application prospect in slope protection and ecological construction.However,the hydration reaction of portland cement,the core cementitious material of vegetation concrete,result in an alkaline soil and water environment in the substrate,and limits the development and metabolism of vegetation and microorganisms on slope,ultimately limit the development of slope ecosystem.Arbuscular mycorrhizal（AM）fungi,as a common material for ecological restoration,play a significant role in the restoration of damaged ecosystems.In this experiment,glomus mossea（GM）and glomus intraradices（GI）were used as inoculants,festuca elata and cassia glauca were used as host plants,and cement content and inoculation method were considered as varying factor to explore the effect of exogenous AM fungi inoculation on plant growth,microbial activity and nutrients in substrate of vegetation concrete ecological protection technology.The following conclusions are drawn:The maximum infection rate of GM and MI to festuca elata was under 5-8%cement content,and that of GI was under 5-10%cement content.The cement content had no significant effect on the infection to cassia glauca.For festuca elata,GM increased seed germination rate,intercellular CO₂concentration,soil microbial biomass C,microbial biomass N,microbial biomass P,total N,alkaline N content under the full cement content;increased stomatal conductance and available P content under the low cement content;increased plant height,root shoot ratio,net photosynthetic rate and reduced substrate p H under the high cement content.GI increased seed germination rate,intercellular CO₂ concentration,urease activity,microbial biomass C,microbial biomass N,microbial biomass P,alkaline N and available P content under the full cement concentration;increased total N content under the low cement content;increased plant height and reduced substrate p H under the high cement content.Mi increased intercellular CO₂ concentration,microbial biomass N,microbial biomass P,alkaline N and available P content under the full cement content;increased plant height under the low cement content,increased seed germination rate,root shoot ratio,urease activity and reduced substrate p H under the high cement content.For cassia glauca,GM increased seed germination rate,urease activity,microbial biomass N,microbial biomass P,available P content and reduced substrate p H under the full cement content;increased net photosynthetic rate,invertase activity and microbial biomass C content under the low cement content;increased plant height,stomatal conductance,intercellular CO₂ concentration,transpiration rate,organic matter and Total N content.GI increased plant height,intercellular CO₂ concentration,phosphatase activity,microbial biomass N,microbial biomass P,alkaline nitrogen,available phosphorus content and reduced substrate p H under the full cement content;increased seed germination rate,root shoot ratio,net photosynthetic rate,transpiration rate and microbial biomass C content under the low cement content;increased stomatal conductance,organic matter and total N content under the high cement content.MI increased phosphatase activity,microbial biomass N,microbial biomass P,alkaline nitrogen content and reduced substrate p H under the whole cement content;increased root shoot ratio,microbial biomass C,organic matter and available P content under the low cement content;increased leaf number,stomatal conductance,transpiration rate and urease activity under the high cement content.For the optimal inoculation scheme:the single inoculation of GM should be selected in order to improve the vegetative growth and substrate characteristics of festuca elata and cassia glauca in vegetation concrete.