Effect Of UV-C Treatment On Chilling Injury Of Postharvest Green Pepper Fruit

Green pepper(Capsicum frutescens L)is widely cultivated all over the country,and it is one of the important bulk vegetables in our country and is deeply loved by consumers.Refrigeration is currently one of the main methods adopted in the process of postharvest storage and preservation of green peppers.However,because green peppers are more sensitive to low temperatures,they are prone to chilling damage during cold storage,resulting in serious degradation of green pepper fruit quality.In order to suppress the chilling injury of post-harvest green peppers,0.25 k J/m~2 short-wave ultraviolet(UV-C)was used to treat the round green pepper"Toyota Rookie",and the effect of UV-C treatment on the chilling injury of green peppers was studied.The results show that 0.25 k J/m2UV-C treatment can effectively reduce the chilling injury index of green peppers,reduce the weight loss rate,reduce the damage of cell membranes,slow down the hardness decrease,and alleviate the reduction of soluble solids,chlorophyll and vitamin C content during the cold storage of green peppers.Effectively maintain the good commercial value and edible quality of green pepper fruit.At the same time,it was found that UV-C treatment can effectively inhibit the decline of ascorbate peroxidase(APX)activity during cold storage of green peppers,enhance the activities of catalase(CAT)and peroxidase(POD),and improve the chilled green peppers The fruit’s stress resistance effectively extends its shelf life.At the same time,in order to explore the molecular mechanism of UV-C treatment on green pepper chilling injury,a full-transcriptome analysis was performed using high-throughput sequencing technology,and a total of 2,000lnc RNAs were identified from UV-C treated and untreated chilled green pepper fruits,35 Circ RNA,18 mi RNA and 5190 m RNA were differentially expressed.Through GO(Gene Ontology),the biological processes,cellular components and molecular functions of differentially expressed genes mainly enriched were analyzed.KEGG(Kyoto Encyclopedia of Genes and Genomes)enrichment analysis found that these differentially expressed RNAs were mainly enriched in metabolic pathways such as glutathione metabolism,fatty acid degradation,plant hormone signal transduction,starch and sucrose metabolism,and amino acid biosynthesis.Specific analysis of enzymes and transcription factors for differentially expressed RNA function prediction design.At the same time,an interaction RNA network related to the chilling injury of green pepper fruits by UV-C treatment was constructed,and four competitive endogenous RNA networks were found,which provided insights for the further research on the molecular mechanism of chilling injury of green pepper under the influence of UV-C.