Effects Of Application Process Of Microbial Fertilizer On Growth And The Structure Of Rhizosphere Microbial Community Structure Of Continuous Peanut

Peanut is an important agricultural crop in China,but the relative concentration of peanut production areas and farmers’ planting habits cause peanut long-term continuous cropping,led to rising series of problems.With the increase of continuous cropping years,the continuous cropping obstacles of peanut are more and more serious,which mainly include the decrease of peanut yield,the occurrence of soil-borne diseases and the change of soil microbial environment.In recent years,due to the outstanding advantages such as improving soil microbial environment and alleviating continuous cropping obstacles,microbial fertilizers have attracted the attention of a large number of scholars.This experiment was designed to find the optimal application mode of microbial fertilizer by comparing the effects of different application modes of microbial fertilizer on peanut growth,including soil enzyme activity and soil microbial community structure.The results of this experiment are as follows:1.Effects of different fertilization methods on peanut growth: Compared with treatment CF,treatment SDA,treatment MEA and treatment SDA+MEA all improved the photosynthetic efficiency and chlorophyll content of peanut.The contents of total phosphorus,total nitrogen,total potassium and calcium and magnesium in peanut plants were increased.The activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)in peanut plants were increased,the root activity of peanut was increased,malondialdehyde(MDA)content in peanut plants was decreased,and the yield of peanut was increased.In the mature stage,the net photosynthetic rate of SDA treatment was 19.34 mol·m-2· S-1,and that of CF treatment was 9.56 mol·m-2· S-1,which was 1.02 times higher than that of CF treatment.Significant difference was formed between the two treatments.SDA treatment also improved SOD and POD enzyme activities more significantly,and SDA treatment increased SOD activity by 18.85% compared with CF treatment at POD stage.Compared with CF treatment,POD enzyme activity was increased by 20.49%,forming a significant difference.MDA content in plants treated with CF at antependium was increased by 71.50%,68.29%,and 1.14 times compared with that treated with SDA,forming significant differences.The root activity was also the best,which was significantly different from CF treatment.The yield of peanut under SDA+MEA treatment was the highest,432.04 kg/mu,which was 15.26% higher than that under CF treatment,with a significant difference.Effects of different fertilization methods on soil enzyme activity.2.Effects of different fertilization methods on soil enzyme activity Compared with CF treatment,SDA treatment,MEA treatment and SDA+MEA treatment all improved the activities of urease,catalase and acid phosphatase in peanut soil.Among the three treatments,SDA+MEA increased catalase and acid phosphatase activity more significantly,the catalase activity in the peanuting stage was 88.89% higher than that in the CF treatment,and the catalase activity in the pod stage was three times higher than that in the CF treatment.SDA+MEA increased 20.35% in the anther stage compared with CF treatment,and 47.47% in the pod stage compared with CF treatment,forming a significant difference with CF treatment.MEA treatment had the best effect on the improvement of peanut soil urease activity,42.39% higher than CF treatment at peanuting stage,40.63% higher than CF treatment at pod stage and significant difference from CF treatment.3.Effects of different fertilization methods on soil microbial community structure Compared with CF treatment,SDA treatment,MEA treatment and SDA+MEA treatment all improved the diversity of bacteria in the soil microbes in the rhizobacteria.Actinobacteria,Bacteroidetes,Saccharibacteria Firmicutes,Planctomycetes,Verrucomicrobia,Armatimonadetes and FBP increase the abundance of bacteria under the influence of biological fertilizer.Chloroflexi,Nitrospirae,Parcubacteria and Latescibacteria the abundance of Proteobacteria,Acidobacteria,Gemmatimonadetes,Chloroflexi,Nitrospirae,Parcubacteria and Latescibacteria has decreased under the influence of biological fertilizer.At the genera level,the relative abundance of Mucilaginibacter in SDA treatment increased by 6 times in the first 10 dominant genera.Relative abundance of Leucobacter obacter was increased by 11.18.The relative abundance of Pseudoclavibacter increased 5.84 times during SDA treatment.The application of microbial fertilizer significantly affected the abundance of bacteria in the rooting zone of peanuts.The abundance of fungi was reduced in all three treatments.The ascomycetes Ascomycota,Zygomycota,Chytridiomycota and Glomeromycota lower relative abundance.The abundance of Basidiomycota and Rozellomycota increased after the application of biotrophic fertilizer.At the level of genus,Fusarium was reduced by 90.84% in SDA+MEA treatment.Pseudogymnoascus reduced by 82.78% in SDA+MEA treatment.Cryptococcus decreased by 77.69% in SDA+MEA processsing.The relative abundance of Glomus and Scleroderma decreased to 0.Application of biological fertilizer significantly reduced the fungal diversity of the rhizosphere soil.The microbial community structure,species abundance and diversity of rhizosphere soil were significantly different from that of conventional fertilization.