| In this experiment,two-way DNA sequencing and time-of-flight mass spectrometry were used to analyze the polymorphisms of MyoG and Myf5 genes in 434 sheep from the F2 generation of Texel×Kazakh crosses and their correlation with carcass traits.Explore the effects of MyoG and Myf5 genes on the carcass development of Texel×Kazakh cross F2 generation,and screen out candidate genes and molecular markers that have important effects on carcass development,providing an important reference for the genetic breeding of Texel×Kazakh cross F2 generation.1.Association analysis of MyoG gene polymorphism and carcass traitsA total of 4 SNPs in MyoG gene were found to be related to carcass traits,located on introns,all in a Hardy-Weinberg equilibrium state.Among them,rs407552631 and rs400160301 belong to moderate polymorphism,and rs600781279 and rs410212255 belong to low polymorphism.In the genotype effect of rs600781279,the ankle width of GG genotype individuals was extremely significantly higher than that of AG genotype individuals(P<0.01).In the genotype effect of rs407552631,the shoulder width of GT genotype individuals was significantly higher than that of TT genotype individuals(P<0.01),and the carcass length,ankle width and hip width of GT genotype individuals were significantly higher than those of TT genotype individuals(P<0.05).In the genotype effect of rs410212255,the chest depth of GA genotype individuals was significantly higher than that of GG genotype individuals(P<0.05).In the genotype effect of rs400160301,the hip width of GG genotype individuals was extremely significantly higher than that of AA genotype individuals(P<0.01),and the carcass length of GA genotype individuals was significantly higher than that of AA genotype individuals(P<0.05).GG gene The shoulder width and chest depth of the AA genotype individuals were significantly higher than that of the AA genotype individuals(P<0.05),and the ankle and hip widths of the GA genotype individuals were significantly higher than those of the AA genotype individuals(P<0.01).2.Association analysis of Myf5 gene polymorphism and carcass traitsA total of 3 SNPs in Myf5 gene were found to be related to carcass traits.Among them,rs416158998 is not in Hardy-Weinberg equilibrium,and the remaining 2 sites are in Hardy-Weinberg equilibrium.A116460176 G is a missense mutation from alanine to threonine,rs412277497 is a missense mutation from serine to cysteine,and the remaining two SNP sites are intron mutations.rs416158998 and rs412277497 are moderately polymorphic,and A116460176 G and rs588855095 are low-polymorphism.In the genotype effect of rs416158998,the chest width of individuals with CC genotype was extremely significantly higher than that of individuals with CT and TT genotypes(P<0.01),and the chest width of individuals with CT genotype was extremely significantly higher than that of individuals with TT genotype(P<0.01),the shoulder width of CC genotype individuals was significantly higher than that of CT genotype individuals(P<0.05),the shoulder width of CT genotype individuals was significantly higher than that of TT genotype individuals(P<0.05),the shoulder width of CC genotype individuals was extremely It was significantly higher than that of TT genotype individuals(P<0.01),and the hip width of CC genotype individuals was significantly higher than that of TT genotype individuals(P<0.05).In the genotype effect of rs588855095,the hip width of GA genotype individuals was significantly higher than that of GG genotype individuals(P<0.05).In the genotype effect of rs412277497,the breast width of GG genotype individuals was significantly higher than that of CG genotype individuals(P<0.05),and the breast width of CG genotype individuals was extremely significantly higher than that of CC genotype individuals(P<0.01).The carcass length,chest width and shoulder width of genotype individuals were significantly higher than those of CC genotype individuals(P<0.05). |
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