| Molecular marker technology is used to study the sequence differences of DNA among different organisms,so as to understand and distinguish individuals.According to the usage of molecular markers,molecular markers can be divided into four categories.SSR technology is based on PCR molecular markers,which has high polymorphism and can distribute the whole genome;simple banding,simple analysis and time-consuming;good co-dominance,which can be used to identify heterozygous individuals;simple operation,good repeatability and stability.SSR markers are widely used in DNA fingerprint construction,germplasm identification,molecular assisted breeding and other fields.In order to obtain an SSR marker method for identification of ’Yellow Rose’ in non-heading Chinese cabbage.In this study,59 pairs of molecular marker primers were amplified by PCR and then electrophoresed on 8%polyacrylamide gel.After analyzing the electrophoretic bands,molecular marker primers which can distinguish the three varieties of oil ’Aijiaohuang’,’Yellow Rose’and ’Lvxing’in Non-heading Chinese Cabbage were selected.The results showed that SSR03118 primers showed polymorphism in the gel electrophoresis map of the three varieties.This method does not require a large number of cultivated plants and is not affected by the growth state of the plant.It has the advantages of rapid,accurate detection,time and labor saving.It is worthwhile to promote effective supervision and arbitration when there are fakes or inferior varieties or controversial situations.In the process of plant evolution,some plants rely on the multiplication of their genomes to complete evolution.In actual production,the chromosomes of plant cells are doubled by artificial mutagenesis to obtain high quality germplasm resources and achieve multiple sports species.In order to obtain the tetraploid plants of non-heading Chinese cabbage,the ’Yellow Rose’ was used as the test material,and the growth point of the seedlings was dripped with aqueous solution of Oryzalin.The concentrations were 0.06 mmol·L-1,0.08 mmol·L-1,0.10 mmol·L-1,and the test material were treated five times respectively.During the growth of the plant,the autotetraploid non-heading Chinese cabbage was continuously identified and screened.The nutritional quality of diploid and tetraploid was compared after the identification.When the concentration was 0.08 mmol·L-1,the aqueous solution of was the best in the treatment of ’Yellow Rose’,and the tetraploid induction rate was 6.60%.Morphologically,compared with diploid plants,the tetraploid plants showed a tendency to become larger include the whole plant,leaves,flowers,seed pods and seeds;anatomically,the tetraploid plants had larger pores and lower density,and the pollen grains were irregularly shaped and more rounded and full;cytologically,the number of diploid chromosomes was 20 and the number of tetraploid chromosomes was 40;the results of flow cytometry showed that the fluorescence intensity of tetraploid DNA was about twice that of diploid.Comparison of nutritional quality between diploid and tetraploid plants,the contents of chlorophyll,soluble protein and soluble sugar in tetraploid plants have greatly improved.However,cellulose and nitrate nitrogen content were lower than diploid plants,respectively.The plant quality,ten leaf thickness,leaf width,petiole length,petiole width and petiole thickness of tetraploid plants were greatly improved,while plant height and leaf length were not significantly different from diploid.Successfully screened out the optimum concentration of Oryzalin for mutagenesis of ’Yellow Rose’,and obtained autotetraploid with richer nutritional quality. |
PDF Full Text Request