Genetic Localization And Breeding Value Assessment Of Soybean Male Sterility Mutant 5053

Male sterile mutant is an important germplasm for hybrid vigor utilization and recurrent selection in soybean.A male sterile mutant,ms5053,was obtained by mutagenesis of Jidou17 with Ethyl Methane sulfonate（EMS）.Some seeds could be occasionally found on male sterile plants,which meaned this mutant might be used in recurrent selection in soybean breeding.In order to utilize this mutant in future,the gene mapping and genetic stability of this mutation gene,and the pod number on male sterile plants_msPNunder different genetic backgrounds was studied in this thesis.The genetic characteristics of this mutant was assessed by Chi-square test with M4～M8and M12segregation populations.Gene mapping was carried out by 200K SNP（Single Nucleotide Polymorphism）microarray combined with BSA（Bulked Segregant Analysis）method.Genetic stability was assessed by multiple years and two locations.The breeding value of this mutant was measured with several populations which derived from some naturally pollinated seeds.The results showed that this mutation phenotype was controlled by a pair of recessive genes and possessed a good genetic stability across different years and locations.The mutation gene was primarily mapped between SNP Gm01＿29521777and Gm01＿35097697 on Chromosome1 with a 5.6 Mb interval.The genetic character of this mutation gene varied from population to population.In some populations,the male sterility was controlled by one gene,while by two genes in some other populations.Planting date might also have some role on the expression of mutation gene,because the male sterility was controlled by one gene when planted early but by two genes when plant mid and late in F3population of natural crossing combination 1513.In 2017,there were 0to 28 pods per male sterile plant with a mean of 2.73 pods per male sterile plant in normal field.The ms PN varied significantly among genetic backgrounds and planting dates.The ms PN was significantly higher in F2population derived from natural crossing combination1517 than from the naturally pollinated crossing 1514,but not other natural crossing combinations at P=0.05 level.The ms PN was significantly lower when planted late than planted early and mediately.The results in this thesis would set a solid foundation for gene cloning and its utilization in future.</sub>