Function Research Of The Transcription Factor FTF1 Of Fusarium Oxysporum F.sp.cubense

Fusarium wilt of banana caused by Fusarium oxysporum f.sp.cubense is a serious threat to the development of banana planting.The pathogen invades from the wound site of the root,colonizes and secretes toxins in the vascular and blocks it.The host died of wilting because it could not normally absorb and transport the substances.At present,the main control methods of the disease are breeding resistant varieties,chemical drug control,biological control,etc.,but the effect is not ideal.To study the pathogenicity molecular mechanism of Foc can provide theoretical guidance for the control of Fusarium wilt.The transcription factor FTF1 is closely related to the pathogenicity of Foc,but its regulatory mechanism is not clear.In this study,the mechanism of FTF1 in regulation of Foc TR4 was studied in the following two aspects:1.Predicting the downstream target protein of FTF1 by proteomics and bioinformatics: constructing the over expression strain of FTF1,and simulate the state of pathogen infection with liquid tsarist medium to induce protein secreting.And then,secreted proteins were identified by label-free proteomics and bioinformatics.Finally,74 candidate effector proteins are regulated by FTF1,and 27 candidate effector proteins are directly regulated by FTF1.2.Knock-out FTF1: in Foc,being multiple copies,FTF1 can not be knocked-out by traditional methods.In this study,CRISPR/Cas9 system was established to lay a foundation for the knockout of FTF1.In this study,we preliminarily studied the function of the transcription factor FTF1,which provides a theoretical basis for clarifying the mechanism of FTF1 regulating Foc TR4,and provides a new idea for the control of Fusarium wilt.