Mechanism Research Of Baicalin Inhibiting Biofilm Formation Of Escherichia Coli

Bacterial infections are extremely prevalent in clinical cases,and bacterial resistance becomes more severe over time.Antibiotics cannot completely kill resistant bacteria at normal doses.Biofilm is composed of a single or multiple population cluster and its hydration for the extracellular matrix of polymer matrix,the maturity of the bacterial biofilm has the potential of larger environment under pressure,they can provide a physical barrier to protect the living cells,the presence of biofilm bacteria to drugs increased tolerance of 600-1000 times,and biofilm mediated bacterial infection is persistence,repeated infection more,it makes the antibiotic treatment more difficult.It is urgent to find effective anti-biological agents.From the perspective of the regulation of signal molecules,this study explored ways to reduce the invasiveness of Escherichia coli.Research using the transcriptome sequencing technology to the amount of baicalin treated Escherichia coli gene expression changes of combination of baicalin treated Escherichia coli biofilm inhibition of biofilm formation in the standard model,build up the baicalin in Escherichia coli mechanisms underlying regulation network,the test results show that the subinhibitory concentration of baicalin can significantly inhibit Escherichia coli biofilm formation,the main regulatory mechanism for through inhibition of Escherichia coli system of type I flagellum the expression of related genes,which reduces the Escherichia coli adhesion ability and the ability and the ability to migrate,the E.coli invade a significant reduction in the ability.Study 1:Effect of baicalin concentration on the formation of Escherichia coli biofilm.1.The standard model of biofilm formation of Escherichia coli was established and the crystal violet staining scheme was determined.The standard model showed that the Escherichia coli and the standard Escherichia coli strain ATCC25922 were both strains with strong film-forming ability.2.The minimum inhibitory concentration of baicalin on Escherichia coli was tested,and the killing and inhibiting effects of a single component of traditional Chinese medicine extract on bacteria were verified.3.The results showed that baicalin could significantly inhibit the formation of Escherichia coli biofilm and the initial adhesion of bacteria on abiotic surface under the concentration of subinhibitory bacteria.Stady2:Identification of intracellular differential genes and screening of biofilm related genes after baicalin treatment of Escherichia coli.1.The mRNA transcription libraries of Escherchia coli in the drug-free control group and the baicalin-treated group were constructed,and 30213934 and 35390308 clean reads were obtained,respectively.A total of 97 differentially expressed genes were obtained by differential expression analysis,among which 38 were significantly up-regulated and 59 were significantly down-regulated after baicalin-treated.2.GO analysis and KEGG enrichment analysis of differentially expressed genes showed that these genes were significantly enriched in three aspects:biological process,cell composition and molecular function.3.In choosing projections for significant difference of gene and bioinformantics analysis found that baicalin effect on Escherichia coli is the most significant aspect of focus on related gene type I flagella,and type I flagella system is a key factor of bacteria cell adhesion,hints of baicalin inhibition of Escherichia coli biofilm formation mechanism may be to curb its initial cell adhesion.