Screening Of SNP Core Loci And System Scheme For Purity Identification Of Maize Hybrids

With the rapid development of China’s maize industry,the quality of maize seeds has been paid more and more attention.Purity is one of important indexes of maize seed quality,especially for inbred seedling in hybrids,which is the key factor affecting field yield.With the development of biotechnology,molecular detection technology has been widely used in maize purity identification,but the existing purity identification schemes not only have certain limitations,but also have elevated space for further improvement in efficiency and convenience.This study relies on the advantages of co-dominant,high stability,and dimorphism with SNP markers.Furthermore,KASP technology platform has the advantages of high throughput,low cost,high automation,and few steps,which the combination of these two technology is very suitable for seed purity testing of maize.The DNA rapid extraction method and PCR system has been optimized in this study,it can be better applied to quickly purity identification,and it also promotes the identification scheme to be more convenient.In this research,60 candidate loci were selected from 384 SNP basic loci,which were converted into KASP primers,and 95%of the loci were successfully transformed.Comprehensive consideration of multiple indicators such as loci parental complementary rate,polymorphism,stability and genotyping effects,20 loci were selected as the core loci of maize hybrid purity identification,which can effectively identify 99.7%of the purity of the test samples.Based on the characteristics of SNP markers compatible with multiple platforms,a high-throughput purity detection scheme is established.When the sample information is known,the standard sample fingerprint library can be queried to obtain the amphiphilic complementary loci.If the sample information is unknown,the purity core loci can be used to quickly establish the sample fingerprint to obtain the amphiphilic complementary loci.In this study,KASP technology was combined with rapid DNA extraction method for rapid purity identification,which has the characteristics of fast,accurate,high throughput and low cost.The research provides a reference for other crops,which can help promote the unified development of China’s multiple crop seed detection levels.The results can provide more schemes for government supervision.