| Setaria italica is one of the important crops in China.However,downy mildew disease of millet occurs from time to time,which seriously affects and harms the yield and quality of millet.At present,the disease is mainly prevented by chemical control.The heavy use of pesticide will cause problems such as pesticide residues and environmental pollution.In order to achieve sustainable agricultural development and improve the ecological environment,it is urgent to seek an efficient,safe and environmentally friendly biological control method.This study used trapping method to separate mycoparasite of oospores from the soil,and to investigate the recovery rate of trapping and burying oospores and mycoparasitic rate of fungi,and preliminary identification of mycoparasite of oospores.The aim is to lay the foundation for the biological control of downy mildew disease of millet.The main results obtained in this study are as follows:1.The oospores were collected from the diseased plant of downy mildew disease of millet,21 trapping and burying sites of oospores were set in the field.After buring,oospores of 3 burying sites were recovered by the saline suspension sieving method every 30 days.The oospores were recovered for 7 times,with an average recovery rate of 54.74%.2.After recovering the trapping and burying oospores and repeating centrifugation every time,separation of mycoparasite by single oospore inoculation,inoculating and separating 7 times in total,with an average mycoparasitic rate of 29.29%.Oospores without trapping and buring were inoculated,with a mycoparasitic rate of 8.67%.A total of 2,400 oospores were inoculated in this study,and 641 mycoparasite of oospores were separated.3.641 mycoparasite of oospores were morphological identified and divided into 5 genus,which are Fusarium,Penicillium,Trichoderma,Alternaria and Pythium,of which 279 are Fusarium,including 26 contrast mycoparasite;168 are Penicillium;117 are Trichoderma;48 are Alternaria;29 are Pythium. |
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