LED is widely used to improve crop yield and quality and is used less on tea plant currently.In the cultivation of tea plant,the LED can be used for supplement light,and the quality of the tea can be improved from the physiological level to achieve a pollution-free and safe,green agriculture.In this study,tea plant were treated by setting three kinds of LED(blue light,red light,purple light),and the tea plant were continuously filled with light for a period of time under the shade greenhouse to explore the effects of different light qualities on the physiological and biochemical characteristics of tea plant.Here is the flowering results:1.Different light quality has obvious influence on the morphological development of tea bud leaves.In the autumn,light supplementation inhibited the growth of tea shoots,and the purple light inhibited the most,which made the leaf shape worse,the growth potential was uneven,and the leaf area decreased.The study showed that the first true leaf and the second true leaf were treated by purple light.Leaf area decreased by 28.25% and 72.99%,respectively.In the spring,light supplementation is conducive to the growth of tea shoots.The purple leaf treatment increased the leaf area of the first true leaf and the second true leaf by 76.72% and 41.62%,respectively,which proved that the light quality of different seasons is different for the growth of tea leaves.The effect of supplementing purple light in spring is more conducive to the growth of tea tree bud leaves and improve tea yield and quality.2.Different light quality had different effects on the antioxidant enzyme activities in tea plants.Blue light,red light and violet light promoted the activity of POD enzyme,which increased by 42.89%,43.03% and 47.56%,respectively.The reaction under light quality was different.The activity of PPO enzyme in red light treatment increased by 67.98%,while the treatment of purple light decreased by 68.65%.All three kinds of light quality inhibited PAL activity.3.Red light can effectively improve the photosynthesis ability of tea plant.After longterm red light treatment,the actual photosynthetic efficiency Y(II)of tea plant increased by 43.75%.The absolute electron transport rate ETR and photochemical quenching of q L and q P in red light treatment increased by 42.73%,33.45%,and 31.58%,respectively.Comparing the effects of three kinds of test light on the light system of tea plant,red light has the best effect,followed by blue light and purple light.4.Different light quality treatment of tea plant can regulate the carbon and nitrogen metabolism of tea plant,confirm that blue light promotes nitrogen metabolism of tea plant,and red light promotes carbon metabolism of tea.After supplementing with blue light,the content of nitrogen metabolism related products in tea plant increased,the free amino acid content increased by 0.32%,and the content of caffeine increased by 0.26%.In the amino acid group analysis,the blue light treatment of theanine increased by 14.29%,while the red light and violet light treatment decreased the theanine content,which decreased by 7.96% and 14.49%,respectively.The level of carbon metabolism in red light treatment did not increase significantly,and the soluble sugar content increased by 1.39%.The EGCG content decreased by 0.98%,while the EGCG content of blue light treatment increased slightly.Therefore,blue light is more conducive to the improvement of tea quality.5.Different light quality treatments significantly affect the changes in related amino acids in the metabolism of theanine.The content of glutamic acid and alanine in blue light treatment increased by 9.67% and 18.18%,respectively,and the re-assimilation of theanine in blue-light treated leaves was promoted.Red light treatment of glutamic acid,glutamine,γ-aminobutyric acid,alanine content decreased by 12.90%,55%,28.86%,27.27%,causing resistance to the re-assimilation of theanine in leaves.The content of glutamic acid in purple light decreased by 9.68%,while the content of alanine increased by 18.18%,which inhibited the transport of theanine. |