Effects Of Nitrogen,Sulfur And Interaction On Growth And Physiological And Biochemical Indexes Of Spring Rape Seedlings

Rational utilization of sulfur fertilizer is very important for spring rape production,because the spring rape needs more sulfur fertilizer.The combination application of N and S could increase the utilization and metabolism of nitrogen and sulfur in crops,and lay a foundation for high crop yield.In order to clarify the physiological and biochemical indexes of spring rape seedlings under different sulfur application,nitrogen application,and nitrogen and sulfur interaction,based on the spring rape seedlings by pot experiment.The results were as follows:（1）In a range of sulfur application(6.60～19.95 mg·kg^-1)could significantly promote POD activity,decreased SOD activity and MDA content in seedling stage of leaves;Improve the content of dry weight in above-ground,chlorophyll a,chlorophyll b and carotenoid.（2）In a range of nitrogen application(95～142.5 mg·kg^-1)the root and leaves growth were significantly promoted,and the content of chlorophyll a,b,carotenoid and soluble sugar were significantly promoted in the nitrogen(95～142.5 mg·kg^-1)condition;In a range of nitrogen application(47.5～95 mg·kg^-1)condition was significantly promote to the accumulation of soluble protein at the later stage of the spring rape seedlings.（3）There was a negative correlation between MDA content and SOD,POD activity,SOD activity was negatively correlated with POD activity at single sulfur in30 d;There was a linear negative correlation between the content of MDA and the activity of POD,the content of MDA was positive correlation with the activity of SOD,and SOD activity was negatively correlated with POD activity at single sulfur in 50 d.SOD activity was negatively correlated with POD activity at single nitrogen in 30 d;The content of MDA was negative correlation with the activity of POD at single nitrogen in 50 d.（4）There was a significant influence on the content of Chlorophyll,MDA,soluble protein and soluble sugar,the activity of SOD,POD and root in a range of nitrogen application 142.5～190mg·kg^-1,sulfur application 13.3～20.0 mg·kg^-1.The variance analysis shows that nitrogen mainly affected the growth and morphology in above-ground;Sulfur had a significant effect on the growth and morphology in underground;nitrogen-sulfur interaction had an effect on all indexes.