Research Of Effect Of Nano-Zinc Oxide On Maize Seedlings Growth And Phytopathogens

In this paper,nano-zinc oxide was introduced into the field of plant micro-fertilizer and disease control.The research focused on the effect of optimal biosynthesized nano-zinc oxide on the growth of maize seedling,antioxidant activity and phytopathogens.The research is mainly carried out in four aspects as follows:(1)Optimizing the synthesis nano-zinc oxide by adjusting the volume of plant extract,Zn(CH3COO)2 concentration,solution pH,and reaction temperature,etc.and the particles were characterized by UV-vis,SEM,and EDX;(2)Determination the effect of nano-zinc oxide on root number,root length,root fresh weight,root dry weight,stem and leaf length,dry weight of stem and stem,and fresh weight of stem and leaf of maize seedlings;(3)Determination the influence of nano zinc oxide on POD,SOD,CAT,and MDA of maize seedlings;(4)Measurement the inhibition activity of nano-zinc oxide on Bipolaria maydis,Setosphaeria turcica,Curvularia lunata,and Fusarium graminearum from inhibition rate and detached inoculation.The results showed that the optimal system for biosynthesis of nano-zinc oxide by bamboo leaves is 10 mL extract,1 mM Zn(CH3COO)2,and temperature 60℃ at pH 6.Nano-zinc oxide promoted the growth of maize seedlings at 50～200 μg/mL.The maize seedlings treated with 200 μg/mL nano-zinc oxide had the strongest antioxidant activity.Nano-zinc oxide had obvious antifungal activity against B.maydis,S.turcica,C.lunata and F.graminearum,and the inhibition rate increased along with the concentration of nano-zinc oxide.Related research can provide new research ideas and methods for maize growth regulation and comprehensive management of plant diseases,and can greatly reduce agricultural pollution caused by chemical fertilizers and pesticides.