Wettability And Biological Properties Of Micro Nano Structures On The Surface Of Anastomotic Nails

With the continuous development of clinical surgical suture technology,stapler has been widely used in surgery to replace the traditional manual suture,improve the suture efficiency,shorten the operation time and reduce the pain of patients.As a key component of stapler,titanium nail plays a role in closing wound.Its biological properties in human body directly affect the effect of wound anastomosis.The results show that the postoperative healing and rehabilitation of patients with stapler is better than manual suture,but there will still be complications caused by anastomotic leakage,anastomotic site infection and so on.Therefore,it is necessary to improve the biological properties of the surface of titanium nail,promote the mutual fusion between tissue cells and titanium nail,accelerate the tissue healing of anastomotic site,and reduce various adverse conditions caused by infection in the process of wound healing.Previous studies have shown that the wettability of implant surface is related to biological properties,and hydrophilic surface is more conducive to cell adhesion and proliferation.At the same time,some studies have found that the surface microstructure can change the wettability of the original material surface,and the microstructure of different shapes and scales can make the material surface show hydrophilic or hydrophobic characteristics.Therefore,this paper proposes to process the microstructure on the surface of titanium nail,and improve its hydrophilicity by increasing the solid-liquid contact area on the surface of titanium nail,so as to improve the biocompatibility of titanium nail surface.This paper includes the following research contents:1.Based on the polygonal structure of tree frog finger end as the bionic design basis of micro texture,combined with the classical wetting theory model,the solid-liquid contact area of polygonal structure of tree frog finger end is analyzed.It is found that the hexagonal structure has the best wetting performance,and the influence of hexagonal structure parameters on its surface wettability is deduced.2.In order to study the influence of side length and spacing of hexagonal bionic micro texture on wettability,the micro texture surface with different side length and spacing is simplified into a two-dimensional plane model,and the contact angle is simulated and analyzed by fluent.The results show that the hydrophilicity of the micro textured surface with smaller side length and spacing is better.3.The bionic micro texture and micro nano composite texture are processed on the surface of titanium by laser processing technology and anodic oxidation method.The contact angle values of different forms of microstructure surfaces are measured by the contact angle measuring instrument.The results show that the bionic micro texture designed in this paper can improve the hydrophilicity of the original titanium surface,The micro nano composite texture can further improve the hydrophilic characteristics on the basis of biomimetic micro texture.4.3T3 mouse fibroblasts were cultured on titanium surface,and then PCR and ckk8 were detected to evaluate the growth and adhesion of cells.Combined with the previous contact angle test results,it is found that the hydrophilicity of titanium surface is positively correlated with the growth and adhesion of cells.