Hardware System Development Of High Resolution Light Field Microscopy

Light field microscopy(LFM)can capture the three-dimensional topography information of microstructures by collecting light field images and subsequent image processing algorithms,which has the advantages of fast measurement speed,no damage to the sample under test and so on.However,the light field acquisition method based on microlens array is at the expense of the spatial resolution.In order to improve the imaging resolution of the light field microscope and to obtain the imaging images with large field of view,this thesis presents a hardware system that combines light field microscopy with the Fourier stacking(FP)algorithm.The main work done in this thesis includes the following four aspects.1,The overall design and module division of LFM combined FP algorithm hardware system.According to the functional requirements of LFM and FP,the hardware system adopts a cage-based microscopy imaging system,which mainly includes imaging optical module,lighting and control module and image acquisition module.2,According to the principles of LFM and FP imaging,combined with the detection of microstructure,the core parameters of each module of the system are designed.Based on this,a system-based imaging optical module is proposed,and a lighting solution combining transmission and reflective illumination is proposed.Components selection including micro-lens array is completed according to system parameter requirements.3,The light field microscopy hardware system of LFM combined with FP method is set up,the system is set up and calibrated,and the software platform of transmission illumination image acquisition is developed.The installation of the light path of the light field microscope was completed by using the imaging characteristics and the basic principles of geometrical optics,and its resolution and depth of field were tested.Based on the Qt framework,a cross-platform image acquisition and control software for Windows and Linux was developed to improve the image acquisition efficiency.4,The micro-nano array light field image acquisition experiments were conducted,the experimental data were processed and analyzed,the light field image acquisition and processing based on the FP method was realized,and the sources of errors in the experiment were discussed and the improvement methods were proposed.The main work of this thesis is to design and build a light field microscope system with enhanced resolution,which has the advantage of imaging transparent and nontransparent objects;and designing a method of program-controlled acquisition of images that can be completed by program control Automatic acquisition,improve image acquisition efficiency.After obtaining the FP algorithm of the collected image,the image with high resolution and large field of view can be simultaneously obtained,which will have broad research prospects in the field of microscopic imaging.