| Celery is a plant of Apiaceae（Umbelliferae）widely planted in China.The phenolic compounds in celery have many physiological functions such as anti-oxidation and hypolipidemic.Moreover,celery leaves are rich in phenols and have strong antioxidant activity.Abamectin nano suspension was prepared by high-speed rotary grinding.The secondary metabolites of celery were deeply studied by metabonomics,which revealed the feasibility of exploring the components of celery through metabonomics technology,and further provided technical support for the quality evaluation of agricultural products.The main conclusions are summarized as follows:（1）The physical and chemical properties of abamectin nano suspension,such as levitation performance,particle size,cold and heat stability,all met the requirements of formula screening.Compared with the commercial preparation,it has a better killing effect on Helicoverpa armigera.In addition,the effects of grinding time,grinding speed and the amount of dispersant on the particle size of the prepared suspension were discussed.The minimum particle size is 1.425±0.000μm under the conditions of grinding time of 2 h,grinding speed of 1800 rpm and 3%addition of dispersant.（2）A method was established for the simultaneous determination of 11 phenolic active components in celery using high performance liquid chromatography（HPLC）in this study.Additionally,the method for chromatographic column,optimization of mobile phase conditions and gradient elution conditions were optimized.The results showed that the linear relationships of the eleven phenolic were good in the range of 0.5-10μg/m L with the correlation coefficient R~2≥0.990.The recoveries were in the range of 71.9%～134.0%,and the relative standard deviations were 0.3%～4.0%at three spiked levels（0.2、0.4 and 1 mg/kg）.The limits of detection（LODs）and limits of quantification（LOQs）were 0.07～0.29 mg/kg and0.20～0.84 mg/kg,respectively.The stability of each active ingredient in the standard solutions was good within 24 h with RSDs<3%.The method was sensitive and accurate,and can be used for comprehensive evaluation of celery quality with multiple indexes.（3）Celery leaves produced defense response under abamectin treatment,which was mainly related to apigenin,quercetin,apigenin7-O-β-D-glucopyranoside,rutinum,kaempferol and apiin.There was significant difference between the 5th day and the 0th day of pesticide treatment.At the same time,it was found that the metabolites increased significantly at 3 days compared with 0 days and 5 days.Pesticide treatment affected the metabolic pathway of phenylalanine ammonia lyase in celery,which showed the difference in the content of phenolic compounds.（4）A total of 55 kinds of volatile metabolites were identified by the detection of volatile compounds in celery samples.The total content range is 0.001μg/g to 5.21μg/g.The types of volatile metabolites in celery leaves were quite different under different pesticide treatments,and the effect of abamectin（EC）was more obvious.Compared with CK,the species and content of volatile compounds in abamectin（EC）were increased,while the volatile metabolites of nano preparation decreased and the content was lower than that of the control.In this study,metabonomics technology was established to study the effects of pesticide changes on individual plants and the reaction mechanism.Under pesticide stress,the secondary metabolic pathway of plants becomes more active,which inhibits the invasion and diffusion of pesticide factors by generating more energy and resistant secondary metabolites. |
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