Electrochemiluminescence Detection And Device Research For Amine Organic Compounds

Many food processing and animal breeding companies have frequently caused the abuse of food additives and animal and plant hormones for profit,which has repeatedly become the focus of attention.People’s living environment is constantly changing under the influence of industry and modernization.The emergence of electrochemiluminescence has brought new hope for the scientific community to explore the direction of trace detection.Modern science uses some advanced instruments and various detection methods to detect trace substances in the human body and the environment in order to determine whether it is healthy.The need for safety.Electrochemiluminescence has high sensitivity and low signal noise.Compared with traditional detection methods,it is more convenient and has certain advantages in detection speed,operation difficulty and cost.In this thesis,electrochemiluminescence is used to detect the hazards of sulfonamide residues,to study a more portable and low-cost timely detection system,which consists of the following three parts:The first chapter summarizes the development of electrochemiluminescence and introduces the luminescence mechanism,including luminol,ruthenium terpyridine and quantumdotelectrochemiluminescence.Thedevelopmentof electrochemiluminescence detection instrument is summarized,and the detection method is introduced.The second chapter is based on the electrochemiluminescence of ruthenium terpyridine,using tripropylamine as a co-reactant to detect sulfonamide residues,using a three-electrode system and electrochemiluminescence workstation to find suitable detection conditions for sulfonamides,using phosphate buffered saline(PBS)with p H=7 As an electrolyte,add quantitative ruthenium terpyridine and 1m M tripropylamine in the solution as a luminescent reagent.Under the best conditions,according to the light signal intensity corresponding to different concentrations,a linear curve is drawn and the corresponding linearity is obtained.Regression equation Y(a.u.)=-5002.97X+18517.47,R~2=0.997,Y is the light intensity corresponding to different concentrations of sulfonamide test solution,X is the logarithmic value of sulfonamide concentration(Lg),the detection range of sulfonamide concentration is5μM-5m M,The detection limit is 0.85μM(S/N=3).In addition,it is proved that this method has high selectivity in detecting sulfonamides in the environment of body fluids,and excludes a variety of amines,and has excellent anti-interference ability.The third chapter optimizes the design of the electrochemiluminescence detection system through two main aspects.First,it records the phenomenon of light generation and preprocesses it into a picture format,and develops an image processing algorithm through Python programming.The algorithm converts the RGB(Red,Green,Blue)value of the image to HSV(Hue,saturation,brightness)value.The brightness value(V)is used to analyze the concentration of the analyte,and it is applied to sulfa detection.The brightness value and the logarithm of the sulfa concentration have a good linear relationship.Image analysis simplifies the ECL analysis process and improves the analysis speed.The results showed that the method successfully detected a sulfa concentration of 5μM.The second is the hardware design of portable equipment,introducing the operation flow to the circuit design and the thesis-based sampling chip combined with the subject group.Finally,the image processing algorithm is applied to the system to realize the electrochemiluminescence test with tripropylamine as the co-reactant.The fourth chapter is the summary and outlook.It summarizes the content of the above-mentioned thesis and points out the existing problems,and finally looks forward to the continued research of the subject direction.