The Fabrication Of Sers Probe With Gap And Its Application In The Detection Of Leukemia

The early diagnosis of leukemia based on optical detection methods is of great significance to improve the survival rate of patients,which can exhibit a high sensitivity.Previous studies have shown that chronic myelogenous leukemia（CML）has a strong correlation with the proteins corresponding to BCR-ABL fusion gene,which has been regarded as one of the biomarkers of CML.Surface enhanced Raman scattering（SERS）technology has attracted much attention due to its advantages including high sensitivity,high specificity,narrow spectral bandwidth and nondestructive detection,which has been widely used in the biomedical detection and analysis fields.In the detection process,it is very important to prepare high sensitive SERS probes to obtain stable SERS signal.Among the various kinds of SERS probes,those based on core-shell nanostructures consisting precious metal elements（gold,silver,etc.）have been widely employed.In this paper,first,a novel type of SERS probe with a gap between the metal core and shelllayer was proposed,whose structure and SERS optical characteristics were investigated.Second,by combining the as-prepared SERS probes and magnetic substrate,a SERS detection platform was constructed,which can realize the detection of BCR-ABL fusion protein with a high sensitivity.The platform is based on a"sandwich"immunoassay structure,in which the anti-BCR antibody-modified gold coated Fe₃O₄ nanoparticles were used as the magnetic substrate,while gold@silver core-shell nanorods with a gap structure were used as SERS probes.Specifically,in such a gap type SERS probe,cysteine molecules were connected to the gold nanorods to generate the gap between core and shell,followed by the coating of a silver shell.The surfaces of the probes were modified with Raman reporter as4-MBA and anti-ABL antibodies.Third,the BCR-ABL fusion protein was extracted from K-562 cell lysate for obtaining the calibration curve,whose minimum detection limit was0.05%.Finally,the blood samples of leukemia patients were used for detection.The experimental results proved that the SERS platform was highly specific to BCR-ABL positive blood samples.Such a SERS platform can provide a novel way for the early detection and prognosis evaluation of chronic myeloid leukemia.