Construction And Performance Research Of Sensor Array Based On Carbon Dots

As a sensor element,carbon dots（CDs）have unique optical properties and adjustable surface properties,and are widely used in many fields.The sensor array has the advantages of wide response,high sensitivity,and rich output signals,and can be used for simultaneous detection of multiple analytes.In this paper,three N-doped CDs are used as synthetic objects to construct a fluorescent sensor array to study the recognition performance of the sensor array for tyrosine metabolites;use CDs1 and CDs2 and arginine to construct a sensor array to explore the pairing of sensor arrays.Metal ion recognition characteristics.The main research work is as follows:（1）Preparation and characterization of CDs:synthesis of CDs1 and CDs2 by hydrothermal method,and synthesis of CDs3 by microwave method;by ultraviolet spectroscopy,fluorescence spectroscopy,Fourier infrared spectroscopy and X-ray photoelectron spectroscopy and other characterization methods The chemical composition of each CDs was analyzed,and the results showed that the three CDs were successfully doped with N atoms,and the surface was rich in functional groups such as amino and hydroxyl.（2）Recognition of tyrosine metabolites by sensor array:investigate the specific recognition and response behavior of tyrosine metabolites to different CDs.Combining PCA and cluster analysis,the sensor array can accurately identify 4 tyrosine metabolites（L-DOPA,DOPAC,NE,E）,indicating that the sensor array can detect tyrosine in the concentration range of 1～500μmol/L.Qualitative analysis of acid metabolites,the detection limit is 1μmol/L.Linear fitting is performed on L-DOPA（30.00～500.00μmol/L）,DOPAC（0.00～500.00μmol/L）,NE（30.00～500.00μmol/L）,E（0.00～500.00μmol/L）,and the linear relationship is Good,indicating that the fluorescence sensor array can quantitatively analyze the analyte.In actual samples,the ability of the sensor array to recognize tyrosine metabolites is studied.The concentration of 60μmol/L and above can be accurately identified,with an accuracy rate of 100%,and a good linear relationship with DOPAC,NE,and E.It decays exponentially to L-DOPA and has a good fitting effect.The sensor array can identify and identify tyrosine metabolites of different concentrations in human urine.（3）Recognition of metal ions by sensor array:To investigate the specific recognition and response behavior of CDs sensor array to different metal ions.Combined with PCA and cluster analysis,the sensor array can accurately identify 6 metal ions(Cu²⁺,Fe³⁺,Cr⁶⁺,Fe²⁺,Co²⁺,Ni²⁺),with a detection limit of 0.12 mmol/L.The quantitative analysis results showed that Cu²⁺（0.80～5.60 mmol/L）,Fe³⁺（0.12～5.60 mmol/L）,Cr⁶⁺（0.064～5.60 mmol/L）,Fe²⁺（1.60～5.60 mmol/L）,Co²⁺（0.50～5.60）mmol/L),Ni²⁺（0.80～5.60 mmol/L）are linearly related,R² is greater than 0.94,the sensor array can quantitatively analyze Cu²⁺,Fe³⁺,Cr⁶⁺,Fe²⁺,Co²⁺,Ni²⁺.In PCA,the sensor array can identify 4 different sources of water,indicating that the sensor array has a good potential for development in water pollution and water quality monitoring.In summary,three CDs were successfully synthesized and two sensor arrays were constructed to realize the identification and detection of tyrosine metabolites and metal ions,which performed well in both qualitative and quantitative analysis,and had broad application prospects.