Ratio Flourescence Probe Synthesis And Cells Imageing Based On Indolizine Flourescence Groupe

At present,fluorescent probes have attracted wide attention because of their simple design,convenient synthesis and low cost.Fluorescent probe imaging has the advantages of real-time in situ visualization detection,less damage to detection samples and good reprod-ucibility.It has been widely used in clinical diagnosis,pharmacology,pathology,biochem-istry and other fields.But most fluorescent probes are single signal fluorescent probes.Although it can be detected quickly,it will be affected by the intensity of light source,temperature,instrument sensitivity,probe concentration and environmental conditions,thus reducing the accuracy of detection.Ratio-type fluorescent probes exhibit their unique characteristics by changing the ratio of two or more signal intensities.The fluorescence ratio is not affected by the intensity of the light source,the sensitivity of the instrument,the concentration of the probe and the environmental conditions,thus significantly improving the accuracy of the detection.At present,there are few fluorescent groups used to design and synthesize ratio fluorescent probes,such as rhodamine,coumarin,BODIPY,1,8-naphthalimide,etc.In the early stage,our group designed and synthesized a new fluorescent group,which has good biological activity and optical properties,high fluorescence efficiency and good energy transfer efficiency with donors.In order to study the optical properties of indene compounds in depth,have assembled a ratio fluorescent probe using indene as fluorescent group.The structure was verified by a series of characterization methods and the optical properties were studied.The details are as follows:The first chapter summarizes the research progress and current situation of small molecule fluorescent probes,and the research status of indene.In second chapter 2,designed and synthesized a novel ratio fluorescence probe RX-1based on the mechanism of fluorescence resonance energy transfer（FRET）.The energy transfer efficiency of the probe is 87.3%.The detection limit of Cu²⁺is as low as 9.1 n M.The probe has excellent selectivity and anti-interference ability.In addition,it can also be used for imaging in living cells.In third chapter,designed and synthesized a novel ratio fluorescence probe RX-2based on the mechanism of fluorescence resonance energy transfer（FRET）.The detection limit of the probe for Hg²⁺is as low as 8.76 n M,which has very good selectivity and anti-interference ability.In addition,it can also be used for imaging in living cells.In fourth chaper,designed and synthesized a novel ratio fluorescence probe RX-3based on the fluorophore indolizine was developed.The fluorescent probe has unique fluorescence selectivity,high sensitivity,strong anti-interference ability and low detection limit（30 n M）for SO₃^2-in PBS buffer solution.Most importantly,the probe has been successfully applied to fluorescence imaging of endogenous bisulfite in mitochondria of glioma cells.