Application Of Colorimetric And Fluorescence Probe For Detection Of Antibiotics And ATP Based On Lanthanide Complexes

Antibiotic residues have an important impact on the environment and human health,and ATP content can be used as an important indicator of clinical diagnosis.Therefore,it is of great significance to develop a rapid and convenient method for the detection of residual antibiotics and ATP.However,the current detection methods still have problems,such as the requirement of expensive equipment,professional operators as well as complex synthesis of sensing materials.The large interference of background and relatively single target analysis of the reported work is another challenge.In order to solve the above problems,a colorimetric fluorescence probe based on lanthanide complex was constructed.The multi-target detection was achieved based on the developed single lanthanide complex probe by continuous detection and sensing array.The results are as follows:（1）A colorimetric and fluorescent probe(EBT/Tb³⁺)based on the formation of Eriochrome Black T（EBT）and terbium ion(Tb³⁺)was constructed to realize the multi-mode sequential detection of adenosine triphosphate（ATP）and prulifloxacin（PUFX）.EBT solution is blue,and EBT/Tb³⁺solution is red.Due to the binding ability of ATP to Tb³⁺was stronger than that of EBT and Tb³⁺leading to EBT dissociation with the addition of ATP to EBT/Tb³⁺,and the solution changed from red to blue.After adding PUFX to EBT/Tb³⁺/ATP solution,the color of EBT/Tb³⁺/ATP solution changed from blue to red as well as the emission of Tb³⁺characteristic fluorescence.Based on the above color changes,the sequential detection of ATP and PUFX are achieved by naked eye,UV spectrophotometry and smartphone colorimetry.In addition,the fluorescence detection of PUFX can also be realized.The results showed that the minimum concentrations of ATP and PUFX detected by naked eye were 5.0μmol/L and 10μmol/L,respectively.The detection limits of ATP and PUFX by smartphone colorimetry were3.2μmol/L and 5.0μmol/L,respectively,and the linear ranges were 5.0-150μmol/L and2.5-75μmol/L,respectively.The detection limits and the linear ranges of PUFX by fluorescence were 0.40μmol/L and 2.5-125μmol/L,respectively.The developed method was successfully used to the determination of ATP and PUFX in tap water.The recoveries were94.5%-113%and 89.3%-119.6%,respectively.The relative standard deviations were less than 3.5%and 7.0%,respectively.The developed strategy shows great potential for the low-cost,portable,on-site sensing of ATP and PUFX.（2）A colorimetric and fluorescent probe based on Eriochrome Black T（EBT）and europium(Eu³⁺)complex(EBT/Eu³⁺)was developed for the quantitative and qualitative detection of four tetracyclines（TCs）.As tetracycline（TC）,oxytetracycline（OTC）,chlortetracycline（CTC）and doxycycline（DOX）contain multi carbonyl and hydroxyl groups,their complexation ability with Eu³⁺was stronger than that of EBT,resulting in the free EBT and the color of the solution changes from red to blue.Based on the color change,the quantitative detection of TC,OTC,CTC and DOX by UV spectrophotometry and smartphone colorimetry was realized.In addition,TCs can sensitize Eu³⁺and emitted the characteristic fluorescence of Eu³⁺.Based on the fluorescence and colorimetric signals variation with the structure of TCs in TCs/EBT/Eu³⁺as well as with the help of principal component analysis（PCA）,a sensing array was developed to detect the four TCs.Specially,it can also quantitatively detect TCs as low as 0.50μmol/L and effectively distinguish binary TCs mixture.The method was successfully applied to the identification of unknown TCs in pork and honey samples,and the recognition rate was 100%.