Spectral Study Of Single-degree-of-freedom Dielectrophoresis Under Multi-factor Influence In Microfluidic Field

With the development of biological and medical technology,higher requirements have been placed on the separation,capture and aggregation of biomolecules,so that the accuracy requirements of dielectrophoresis manipulation technology is getting higher and higher.Therefore,as the core of dielectrophoresis manipulation technology,the spectrum of dielectrophoresis has received extensive attention.However,the current research on the spectrum of dielectrophoresis has obvious deficiencies,due to the uneven distribution of(?)|E|~2 in the space and the lack of analysis of the influencing factors in the speed acquisition process,so that the acquired dielectrophoresis speed is inaccurate,resulting in an inaccurate dielectrophoresis spectrum.In order to make up for the shortcomings of traditional methods,a single-degree-of-freedom dielectrophoresis spectrum acquisition method is proposed,so as to obtain a more accurate dielectrophoresis spectrum.This article outlined the current status of the research on dielectrophoresis spectrum at home and abroad,showed the importance of dielectrophoresis spectrum in today’s research,and analyzed the deficiencies in the existing dielectrophoresis spectrum research.In order to make up for the shortcomings of the traditional methods:(?)|E|~2 was uneven in spatial distribution and lacked analysis of the influencing factors in the speed acquisition process,this paper established a single-degree-of-freedom dielectrophoresis occurrence model,and Based on the theory of dielectrophoresis,a spectrum acquisition method for single-degree-of-freedom dielectrophoresis was proposed.In order to improve the generation method of dielectrophoresis in traditional methods,through theoretical analysis,the theoretical conditions for uniform distribution of(?)|E|~2 in a single direction were obtained,thus a single-degree-of-freedom dielectrophoresis generation model was proposed,and particles were realized in a single direction,at the same time,the uniform speed movement was carried out,and the correctness of the single-degree-of-freedom dielectrophoresis generation model was verified by simulation analysis.In the spectrum acquisition method of single-degree-of-freedom dielectrophoresis,with the help of the generation model of single-degree-of-freedom dielectrophoresis,the acquisition mechanism of single-degree-of-freedom dielectrophoresis spectrum acquisition method was explored,and the influence of different factors(spatial distribution of(?)|E|~2,conductivity,electric field frequency and voltage amplitude,fluid flow)on the acquisition of dielectrophoresis speed was analyzed,providing a solid theoretical guarantee for obtaining a more accurate dielectrophoresis speed and dielectrophoresis spectrum.A single-degree-of-freedom dielectrophoresis chip was designed and processed.Experimental parameters,experimental materials and experimental instruments were selected,an experimental platform was set up,an experimental scheme was proposed,and a single-degree-of-freedom dielectrophoresis spectrum acquisition experiment was successfully performed.The effect of the spatial distribution of(?)|E|~2 on the single-degree-of-freedom dielectrophoresis of the particles was verified experimentally;the effect of the flow field flow on the speed of the single-degree-of-freedom dielectrophoresis was verified by experimental video processing and data processing;The method for acquiring the frequency spectrum of electrophoresis realized the elimination of the background noise when acquiring the speed of the single-degree-of-freedom dielectrophoresis,so that the accurate dielectrophoresis speed was obtained.Through the above theoretical analysis and experiments,the accurate single-degree-of-freedom dielectrophoresis speed was obtained,so that the real part of the CM factor with an error range of less than 10%at a specific frequency could be obtained,and the dielectrophoresis spectrum was finally drawn.