Construction And Anti-tumor Performance Of Intelligent Nanogel Drug Delivery System

Cancer has the characteristics of difficult cure,easy to relapse,and high fatality rate.The current traditional treatment methods have certain shortcomings.Among them,chemotherapy,as a common treatment method,has problems such as non-specific recognition,large toxic side effects,and poor drug resistance.And the photothermal and photodynamic therapy of cancer can kill cancer cells in a short time and cause less damage to normal tissues,which has become a research hotspot at the present stage.Therefore,a single treatment method can no longer meet the current needs of cancer treatment,and it is particularly important to find a new type of combined treatment method.The unique physicochemical properties of intelligent responsive nanogels reduce the side effects of drugs,and have a controlled release effect,which are widely used in drug delivery systems.They are commonly used as drug carriers and widely used in drug delivery systems.In addition,nano-carriers can also be used to achieve cancer chemotherapy combined with photothermal/photodynamic therapy to improve the anti-tumor effect.Therefore,the design and construction of a multifunctional nanogel drug carrier are of great significance to the study of anti-tumor effects in vitro.In the study,we designed two intelligent responsive nanogels,loaded with different drugs,and studied the synergistic anti-tumor effects in vitro using the various treatment methods through cell experiments.(1)The temperature-sensitive nanogel(PNIPAM)was prepared by soap-free emulsion polymerization,and then loaded with a large amount of photosensitizer indocyanine green(ICG)and anti-cancer substance 5-fluorouracil(5-Fu),thereby constructing a temperature-sensitive drug delivery system(5-Fu@ICG-PNIPAM)to achieve the chemical-photothermal/photodynamic cotreatment of tumors.The results show that 5-Fu@ICG-PNIPAM has obvious temperature sensitivity,higher drug loading rate,and drug-controlled release ability,which can significantly improve the bioavailability of the drug.In addition,under NIR irradiation,5-Fu@ICG-PNIPAM shows excellent photothermal and photodynamic performance.The cell experiments show that 5-Fu@ICG-PNIPAM is easily endocytosed by He La cells and can produce a large amount of singlet oxygen in the cells.Compared with separate chemotherapy or photothermal/photodynamic therapy,5-Fu@ICG-PNIPAM has a significant decrease in cell survival rate under NIR irradiation,indicating that chemotherapy and photothermal/photodynamic synergistic therapy have excellent anti-tumor properties.Therefore,the temperature-sensitive nanogel drug platform has potential application prospects in anti-tumor therapy.(2)A photo and temperature-responsive nanogel(Au-PDA-PNIPAM)with a core-shell structure loaded with gold nanoparticles was designed,and then the anticancer drug doxorubicin(DOX)was embedded to achieve a tumor combination of chemotherapy and photothermal treatment.The results show that Au-PDA-PNIPAM is a regular-sized nano-sphere,and has high photothermal conversion efficiency and drug loading rate.The cell experiments have proved that NIR irradiation is beneficial to the enrichment of DOX@Au-PDA-PNIPAM in tumor cells and increases the bioavailability of the drug.In addition,it can also be shown that Au-PDA-PNIPAM itself has good biocompatibility,DOX@Au-PDA-PNIPAM has an excellent anti-tumor effect under NIR irradiation,and can increase the mortality of cancer cells through combination therapy.Therefore,the nanogel system has shown great application potential in the collaborative treatment of cancer.