Research On The Detection Of Carbohydrate Compounds By Proportional Fluorescent Gel Fiber

Sugar is a common energy substance,and reasonable intake is very important for the healthy development of the human body.Common sugar substances include glucose,fructose,maltose,etc.among which glucose concentration is an important basis for judging diabetes.Diabetes patients are large in number and widely distributed,and how to achieve accurate and convenient monitoring of glucose concentration has become a research hotspot.At present,there are many household blood glucose meters that can be carried around to facilitate patients to monitor their own blood glucose.Most blood glucose meters are based on electrochemical principles and have certain errors.Researchers continue to seek new directions on the original basis.Judging from the research results,there are two main types.One is to combine with fluorescent dyes to achieve the purpose of detection by changes in fluorescence intensity,wavelength and other properties;the other is to cooperate with hydrogels to achieve detection by changes in its state of contraction and expansion.Single fluorescence is easily affected by the instrument,and the state of the hydrogel is easily affected by the environment(temperature,humidity,etc.),and there is an error problem.This article proposes a segmented gel fiber detection method,which is not only suitable for glucose,but also suitable for other carbohydrates such as fructose.This method is based on a hydrogel system and uses the ratio of the two fluorescences at both ends of the gel as the detection result,which not only reduces the impact of equipment and environment,but also retains the sensitivity and accuracy of fluorescence detection and the biocompatibility and convenience of the hydrogel.The main research contents of this paper are as follows:(1)Analyze the requirements for the detection of gel systems and prepare suitable hydrogels as the detection system.First,adjust the predecessor’s preparation method to obtain a hydrogel with good mechanical properties and transparency.Second,add appropriate amount of fluorescent dye to prepare a uniform and bright fluorescent hydrogel.Third,realize the preparation of segmented gel fibers with obvious segmentation and uniform luminescence.(2)Analyze the detection principle of sugars and explore the detection of concentration in the solution system.First,take glucose,fructose,and maltose as examples to explore the relationship between the p H value and the concentration of carbohydrates,and to understand the change of H~+content in the solution system.Second,choose the appropriate fluorescent dye NPBI1,and choose the solvent according to its properties.Third,take glucose as an example to explore the relationship between the fluorescence intensity of the solution system and the concentration of carbohydrates under the premise that the contents of NPBI1,AAPBA remain unchanged.Fourth,through glucose titration experiments,explore the linear relationship between fluorescence intensity and concentration in a certain concentration interval.(3)Detect carbohydrates with segmented gel system to explore the relationship between ratio fluorescence and concentration.First,by adding carboxyfluorescein and NPBI1 to make segmented luminescent hydrogel fibers with different glucose concentrations to verify whether the segmented gel fluorescence is uniform and bright.Second,by refining the detection unit of carbohydrates,exploring the relationship between fluorescence intensity and concentration under the gel system,and verifying the feasibility of detecting carbohydrates under the gel system.Third,find out the fluorescence linear concentration interval,perform fluorescence detection and corresponding data processing on both ends of the segmented gel,compare single fluorescence and proportional fluorescence detection results,and verify the feasibility of higher accuracy of proportional fluorescence results.