Study On The Therapy Mechanisms Of The Microwave Ablation Of Lung Squamous-cell Carcinoma By Confocal Raman Microspectral Imaging

Confocal Raman Microscopy Imaging(CRMI),as a nondestructive spectroscopic method,can be used to study the "fingerprint" information of substance composition and structure at the molecular level with high sensitivity and specificity.This technique combines confocal imaging with microscopic Raman spectroscopy to accurately measure the biochemical composition of the sample and obtain discrete spectral information from unique spatial locations.This technique has the advantages of rapid,accurate and nondestructive,and has become an important tool for early cancer detection and histopathological biochemical analysis.In this paper,CRMI technology was used to study the carcinogenesis and microwave ablation(MWA)treatment mechanism of lung squamous cell carcinoma(LSCC).The specific work is as follows:Firstly,the principle of various Raman imaging techniques is introduced,and the spectral imaging techniques and data analysis methods used in this paper,as well as the cross-validation of discriminant models and performance evaluation methods are described,and the research scheme and analysis techniques for subsequent work are determined.Secondly,the confocal Raman spectroscopy technique was used to detect the characteristic spectra of human lung tissue and LSCC tissue before and after MWA treatment.Meanwhile,principal component analysis(PCA)and linear discriminant analysis(LDA)were used to establish an effective tissue spectral discrimination model.The results showed that MWA could induce the increase of nucleic acid,tryptophan,phenylalanine,collagen and lipid components in LSCC tissues.In addition,the discriminant model based on PCA-LDA combined with cross-validation achieved an overall classification accuracy of87.6% in the recognition of the three tissue spectra.This study demonstrates the feasibility of confocal Raman spectroscopy in combination with multivariate analysis for histopathological diagnosis and treatment evaluation.Finally,the carcinogenesis mechanism of LSCC tissue and its biochemical response mechanism to MWA treatment were further explored,and the effect of MWA treatment was evaluated.Microscopic imaging of the region of interest was performed by point-by-point scanning,and Raman imaging analysis was performed by univariate combined with PCA and K-means clustering analysis(KCA)in multivariate analysis to reveal the spatial distribution and content differences of different biochemical components.The results showed that the contents of nucleic acid,tryptophan,phenylalanine and other biochemical components in cancerous tissues were increased compared with those in adjacent tissues,while the contents of lipid were significantly decreased..In addition,MWA treatment can induce increased expression of nucleic acid,protein and lipid components in LSCC tissues,and cause changes in protein molecular structure.In this study,the pathological changes of LSCC tissues and the action mechanism of MWA were preliminarily discussed,and the reliability of CRMI technique in the study of histological evolution analysis and treatment evaluation was confirmed.