Chiral Characteristic Of β-Cyclodextrin And Mandelic Acid

This thesis includes following four parts:（1）The chirality,chiral substances,high performance liquid chromatography（HPLC）,optical resolution methods,chiral stationary phases of HPLC,mandelic acid,cyclodextrin,eantioselective membranes etc.were introduced.（2）Usingβ-CD as chiral selector,four kinds of chiral stationary phases were prepared by using three different carbamate and one glycidyl bond arm.With the normal mobile phase CH₃（CH₂）₄CH₃/（CH₃）₂CHOH,the reversed mobile phase CH₃OH/H₂O,the organic mobile phases CH₃OH,C₂H₃N and CH₃OH/C₂H₃N,the optical resolution of 16 racemates was achieved on those chiral columns in HPLC.（3）Synthesizing cellulose tri（3,5-dimethylphenyl carbamate）,it was coated on Si O₂to prepare a chromatographic column.By solubility equilibrium method,the solubility curve of D/L-mandelic acid was ploted.The solubility of D/L-mandelic acid in water,acid and base increased with the increase of temperature.The order of solubility was S_{Na OH}>S_{HCl O4}>S_H2O.（4）Theβ-cyclodextrin-homobenzoyl chloride composite membrane was prepared by interfacial polymerization,and the chiral membrane extraction of D,L-mandelic acid on the membrane was investigated.When the concentration ofβ-cyclodextrin was 0.133 g/m L,the extraction time was 45 min,and the sample concentration was 0.8 mg/m L,the chiral membrane extraction effect of D,L-mandelic acid could reach 59.38%e.e.