| This research introduces the principles of resonance Rayleigh scattering（RRS）and surface-enhanced Raman scattering（SERS）spectroscopic analysis techniques.At the same time,the application progress in environmental pollution and nanocatalysis analysis is studied.It mainly summarizes the preparation methods of covalent organic framework（COF）in recent years,as well as the application and analysis in various fields.The application and characteristics of aptamers in the field of RRS and SERS spectroscopy analysis and other fields are introduced,and the application of aptamers and COF together in the spectroscopic analysis of nanocatalysis has obtained gratifying experimental results and considerable progress.After a brief overview,analysis and comparison of the main measurement methods of glyphosate（GLY）and oxytetracycline（OTC）,the current research is about the COF catalytic reaction system for the determination of trace GLY and OTC in environmental pollutants There are few and many shortcomings,so a simple and sensitive aptamer-mediated COF nanocatalysis-gold nanosol plasma RRS/SERS spectrometric quantitative analysis method was established.Using melamine（Ma）and p-benzaldehyde（Bd）as precursors,a highly catalyzed and stable AuNP covalent organic framework was prepared by polycondensation reaction,and it was characterized by molecular spectroscopy and electron microscopy.AuNP strongly catalyzes the indicator reaction between glycol（GC）and chloroauric acid（HAuCl4）to generate gold nanoparticles（AuNP）,which has a strong RRS effect at 370 nm.After adding Victoria Blue B（VBB）as a molecular probe,it has a SERS effect at 1617 cm-1.Combining AuNP nano-catalyzed amplification reaction and GLY’s highly selective aptamer（Apt）,Apt can be adsorbed and wrapped on AuNP to decrease its activity,thereby weakening its catalytic effect.The AuNP in the system also decreases,that reducing the effects of RRS and SERS.When GLY is added to the system for the test substance,the former specifically binds to Apt to form a stable Apt-GLY complex,which restores surface activity and catalytic effects of AuNP,and AuNP gradually increases,corresponding to RRS And the SERS signal strength is restored.Within a certain range of GLY concentration,the signal intensity showed a linear increase trend.Based on this,a new fast,simple and highly sensitive RRS/SERS method was developed to determine GLY.The linear range of this method for GLY detection is 0.003-0.07 nmol/L,and the detection limit is 0.002 nmol/L.Using this method to analyze GLY in soil samples,the relative standard deviation is 4.78-5.71%,and the recovery rate is 92.3-105.3%.Choosing dicyandiamine（Dd）as the amine group together with terephthalaldehyde（Bd）as the precursor,and the polycondensation of them to prepare a new type of covalent organic framework to DdBd with high catalysis,high stability and a three-dimensional structure skeleton.After being characterized by molecular spectroscopy and electron microscopy,it can be found that DdBd has smaller size particles and stronger catalytic properties.To comparison with other,DdBd can strongly catalyze the indicator reaction between polyethylene glycol 600（PEG）and HAuCl4,and the AuNP produced has a stronger RRS effect at 370 nm.It is effective against Rhodamine6G,Victoria Blue 4R,and Victoria Blue.After comparing blue B,it is found that using VBB as a molecular probe has the strongest SERS effect at 1613 cm-1.This is different from the strongest SERS effect of AuNP at 1617 cm-1.We speculate that the COF particle size causes the blue shift of the spectral peak.Combining DdBd nano-catalyzed amplification reaction and OTC highly selective Apt,due to the functional group between Apt and DdBd and theπ-πsuperimposed hydrophobic group,the surface activity of DdBd is reduced by adsorption and wrapping on the interaction.It Can’t contact with PEG and HAuCl4,and inhibiting the catalytic effect,so that the AuNP in the system is reduced,the RRS and SERS effects are reduced.When OTC is added to the system,the former specifically binds to Apt to form a stable Apt-OTC complex,which restores surface activity and catalytic effect of DdBd,and the reaction rate of the PEG-HAuCl4 system has been accelerated.More and more AuNP makes the RRS and SERS effects gradually increase.And within a certain range,the signal strength increases linearly.Based on this,a new simple highly sensitive and highly catalytic RRS/SERS method was developed for OTC determination.The linear range of this method for OTC detection is 0.0033-0.07 nmol/L,and the detection limit is 0.0009 nmol/L.Using this SERS method to analyze OTC in four soil samples,the relative standard deviation is 1.35-4.78%,and the recovery rate is 94.3-104.9%. |
