Assembly And In Vitro Antitumor Activity Of Fluorescent Magnetic Targeting Drug Loaded Nanoparticles

At present,cancer has become one of the most serious diseases threatening human life and health,causing high morbidity and mortality,and seriously threatening people’s life and health.However,traditional cancer treatment methods,such as surgical radiotherapy,chemotherapy and other methods,have disadvantages such as large side effects and loss of some body functions.In this paper,taking advantage of the many advantages of functional nanomaterials,two magnetic fluorescent drug-loaded nanoparticles with doxorubicin hydrochloride（DOX·HCl）and harmine（HM）as anti-cancer sustained-release drugs were constructed,respectively,and explored The optimal preparation conditions of Fe₃O₄,and the performance of the magnetic fluorescent nano-medicine carrier Fe₃O₄/CTS@CQDs before and after loading were investigated,in order to provide an effective plan for highly targeted drug delivery and in vivo imaging in cancer treatment.（1）According to the analysis of influencing factors,the optimal preparation conditions for Fe₃O₄are the reaction temperature of 80℃,the system p H control at10,the molar ratio of Fe³⁺to Fe²⁺2:1.5,and the average particle size of 19.27 nm;the saturation magnetization is 52.5 emu·g^-1;（2）Biological safety performance research shows that Fe₃O₄/CTS@CQDs based on Fe₃O₄will hardly cause damage to normal human liver cells（HL-7702）through MTT method detection,and basically meet the requirements of biosafety of medical materials;（3）Magnetic performance test results show that the saturation magnetization of Fe₃O₄/CTS@CQDs based on magnetic Fe₃O₄particles is 24.70 emu·g^-1,Fe₃O₄/CTS@CQDs-HM and Fe₃O₄/CTS@CQDs-DOX The saturation magnetization is 21.17 emu·g^-1and 19.96 emu·g^-1,and the residual magnetization and coercivity of the above-mentioned materials are both zero;（4）The particle size test results show that the average particle size of Fe₃O₄/CTS@CQDs is 58.78 nm,and the average particle size of Fe₃O₄/CTS@CQDs-HM and Fe₃O₄/CTS@CQDs-DOX are 91.74 nm and 97.90 nm,respectively;（5）The drug loading capacity experiment results show that Fe₃O₄/CTS@CQDs has a drug loading rate of 18.07%for HM and an encapsulation rate of 20.07%;for DOX·HCl,a drug loading rate of 24.95%and an encapsulation rate of 27.73%;（6）In vitro sustained-release behavior studies have shown that the in vitro sustained-release behavior of Fe₃O₄/CTS@CQDs-HM and Fe₃O₄/CTS@CQDs-DOX follows p H dependence,that is,the lower the p H of the release environment,the higher the drug release rate.（p H=1.2）,the maximum release rate of the drug in 2 h reached 35.55%and 43.03%;（7）The results of blood compatibility studies show that the hemolysis rate of Fe₃O₄/CTS@CQDs-HM is slightly greater than that of Fe₃O₄/CTS@CQDs-DOX,but within a certain concentration range,the hemolysis rate is basically in line with the blood compatibility of medical materials Sexual requirements;（8）The cytotoxicity test results show that both Fe₃O₄/CTS@CQDs-HM and Fe₃O₄/CTS@CQDs-DOX are toxic to normal human liver cells（HL-7702）to a certain extent,and the survival rate of cells corresponding to 25μg/m L They are82.7%and 78.1%.On the other hand,the hepatoma cell（HepG2）is obviously dose-dependent,and the survival rate decreases with the increase of the concentration of the two drug-loaded nanoparticles.The effect is significant,and the survival rate corresponds to 25μg/m L.The rates are only 41.9%and 10.1%;the results of flow cytometry show that the two drug-loaded nanoparticle concentrations can induce apoptosis of liver cancer cells（HepG2）and thus exert anti-tumor effects;（9）Fluorescence imaging results show that Fe₃O₄/CTS@CQDs appears bright green and black with yellowish green light on the surface under a fluorescence microscope,and the fluorescence effect is obvious;two kinds of drug-loaded nanoparticles and liver cancer cells（HepG2）After mixed culture,the drugs can be released from the carrier and enter the cells,making the cells have obvious fluorescence,making them have potential application value in biological research directions such as cell imaging.