| Intensive aquaculture in China is developing rapidly,producing a large amount of steroid estrogens(SEroids)that are excreted from the animal body.Such pollutants enter the surrounding soil and migrate into the water environment.Endocrine disruptors can stimulate or restrain aquatic organisms The function of the endocrine system,which in turn disrupts the stability of the body’s internal environment.The gonad function of aquatic organisms will be abnormal only at a concentration level of ng / L level,posing a greater threat to the ecological environment and human health.Therefore,this estrogen is considered to be an endocrine disruptor,and sewage treatment plants should give priority to controlling emissions.However,bound estrogen(E1-3S),which does not have estrogen vitality,is often produced when it is treated with different free estrogen,and is discharged accordingly.In the sewage treatment equipment system,sulfate-bound estrogen itself does not have a certain interference activity,but in a specific environment,it can be hydrolyzed by arylsulfatase,and then release the free state estrogen with interference activity,that is,it It can be converted into free state estrogen with interference activity controlled by sewage treatment facilities.However,the concentration of bound estrogen discharged into the water body is generally more than half of the concentration of free estrogen.Without special treatment,it may bring inestimable environmental risks.This subject mainly studies on estrone sulfate(E1-3S),which has a relatively stable binding estrogen in the environment and has a high ability to remain in water.The environmental factors that affect the enzyme activity,and then study the effect of arylsulfatase on the removal of estrone sulfate in water and soil environment.Using a controlled laboratory environment for the experiment,this subject studies arylsulfatase and bound estrogen,and explores and concludes:1.The correlation between arylsulfatase activity and the environment under different factors such as different p H,temperature,organic matter and inorganic sulfate,etc.,and carry out orthogonal test.Through the test data,using F test and sig significance analysis,the significance level order of the typical environmental factors is obtained: organic matter content> inorganic sulfate content> temperature,and the best combination of three factors is obtained to maximize the activity of arylsulfatase The value is: temperature 30 ℃,organic matter 0.02 g and sulfate concentration 100 mg / L.2.Under the conditions of the above-mentioned optimal environmental factors,a first-order degradation reaction rate model was established for the conjugated estrogen reaction,the correlation between the reaction rate and enzyme activity was analyzed,and a conclusion was drawn for the study of single-factor organic matter: in a water environment,Arylsulfatase activity decreases with the increase of organic matter content,when the content is certain,it will decrease with the increase of cultivation time,indicating that in the water environment,organic matter has a potential inhibitory effect on arylsulfatase activity;In the soil environment,the activity of arylsulfatase increases with the increase of organic matter content.When the content is certain,it will increase with the cultivation time,indicating that organic matter plays an important role in promoting the activity of arylsulfatase in the soil environment.3.Simulate the ability of arylsulfatase to promote the hydrolysis of estrone sulfate in water environment,and simulate the degradation ability of arylsulfatase to estrone sulfate in soil column in soil environment.Briefly analyze the conversion of the two in different environments and the degradation effect of arylsulfatase on the bound estrogen,and draw the conclusion: under certain environmental conditions,the bound estrogen E1-3S can be arylsulfatase Catalytic hydrolysis releases a certain amount of free estrogen E1.4.Scanning electron microscope SEM and XRD before and after the test were used to characterize the soil particles after sieving and air-drying.It was concluded that the soil material spacing became smaller after the test and the surface of the soil particles became smoother than before.In this study,the typical environmental factors affecting the activity of arylsulfatase enzymes were used to obtain the optimal environmental combination,and the first-order reaction rate analysis was performed to obtain the relationship between the reaction rate and enzyme activity.The determination of the environmental factors provides an effective basis.In the follow-up experiments,the degradation of E1-3S by arylsulfatase and the characteristics of soil changes were explored in water and soil environments.The experimental conclusion verifies that the enzyme has a degradation effect on the target E1-3S,and the research progress on estrogen is more in-depth. |
