Study On Isolation And Identification Of High Temperature Cellulose Degrading Bacteria

China is a big country in agricultural production.The output of straw is increasing.The lignocellulose in the straw is rich in natural resources.Composting is an importand method to dealwith straw.However,the straw is not easily degraded during the process of compost,which puts increasing pressure on resource utilization and environmental protection.At present,the complex microorgnism or single strain with cellulose degrading ability were used to improve the efficiency of cellulose degradation during the process of compost.Compared with the single strain,the microbial community showed stronger stability and degradation ability in cellulose degradation.In this experiment,a microbial community with cellulose degrading ability,named SN-8,was screened from the rice straw compost.The cultivation conditions of SN-8were optimized.The culturable bacteria in SN-8 were isolated by plate culture method followd by the identifacation of species status.On the basis of high-throughput sequencing,the dominant decomposition strains of SN-8 were explored,and the correlationship among different stains were also analysized in combinated with straw degradation rate.The results showed that the cellulose-degrading bacterial community SN-8 was screened,and the degradation rate of straw reached 70% under the conditions at 55℃ and the inoculation rate was 5%.The p H value of the SN-8 shows a tendency to first decrease and then the upper body,and the cellulase activity shows a tendency to increase first and then decrease.On the basis of high-throughput sequencing,the main strain of SN-8 is Bacillaceae,accounted for 31%.In summary,a bacterial community with high-temperature cellulose degradation ability,named SN-8,was isolated which could improve the efficiency of straw degradation in composting,and provide greater potential value for the study of cellulose degradation.