Study On The Detection And Removal Process Of Pesticide Residues In Ginsenoside Extracts

Objective Ginseng is a perennial medicinal material.Pesticides are easily enriched in ginseng during the growth and restricted the development of the ginseng industry.Although traditional removal methods has certain removal effects on pesticide residues,there were some shortcoming have to be consided such as fewer types of pesticide residues removal,low removal efficiency,and secomdary pollution.Based on this,according to the properties of ginsenosides and pesticide residues,this research intended to establish an efficient,economic,safe,and pollution-free method for pesticide residue removal and a fast,efficient and accurate pesticide residues detection method.Methods In this paper,taking ginsenoside extracts as an example,by optimizing the extraction solvent volume,extraction time and the best purification reagent ratio,a Qu ECh ERS-gas chromatography combined with triple quadrupole mass spectrometer pesticide residues detection method was established;Using the difference in the length of time between ginsenosides and pesticide residues in the macroporous resin column and the adsorption effect of the macroporous resin,a method for the removal of pesticide residues in ginsenoside extracts by macroporous resin adsorption was established,and the best removal condition was optimized.In addition to the process according to the differences in molecular weight,molecular volume and hydrophilicity of ginsenosides and pesticide residues,a reversed-phase/hydrophilic two-dimensional liquid chromatography method was established to remove pesticide residues in ginsenoside extracts.Reversed-phase one-dimensional liquid chromatography was used to remove water-soluble impurities,pigments,etc.In the sample and increase the column capacity during the preparation of hydrophilic two-dimensional liquid chromatography.Pesticide residues and ginsenosides were separated on hydrophilic two-dimensional chromatography.The best process for removing pesticide residues in ginsenosides by hydrophilic chromatography was explored in order to achieve the purpose of removing pesticide residues.Results Using gas chromatography-triple quadrupole mass spectrometer to detect the five common pesticide residues in ginseng,the linear correlation coefficient of each pesticide in the concentration range of 5～600 μg/L were higher than 0.994.At3 levels of pesticide residues concentration,the recovery rate ranges from 74.1% to114.9%;Use SP850 type macroporous resin to remove pesticide residues in ginsenoside extracts,and optimize to determine that the optimal loading amount was100%,the loading solution concentration was 40 g/L,and the loading flow rate was 3m L/min.The average removal rate of the next five pesticide residues were over96.33%,and the recovery rate of total ginsenosides were over 81.01%.The ginsenoside extracts samples were prepared by the constructed reversed-phase/hydrophilic two-dimensional chromatography method.It was found that 14 kinds of pesticide residues in the original ginsenoside extract were effectively removed,of which the content of 5 kinds were reduced to below 0.05 mg/kg and 9kinds were completely removed.The recovery rate of total ginsenosides were 88.56%.Conclusion The method of detection of pesticide residues in ginsenoside extracts and two different removal methods were established.The detection method has low background response,high sensitivity,high recovery rate and good stability.The two removal methods established have good removal effects on pesticide residues and high recovery.In the course of the experiment,the solvent used in the two pesticide residues removal methods were environmentally friendly and non-toxic,which were more suitable for industrial production.