| Recent years,Ion mobility technology has become the focus of mass spectrometry application,which has the characteristics of fast analysis speed,high sensitivity,strong applicability,effective reduction of chemical background noise,separation of isomers,etc.And Ion mobility-mass spectrometry(IM-MS)has gradually replaced liquid chromatography-mass spectrometry(LC-MS)as a new standard for detection and recognition of biomolecular molecules.Ion mobility-mass spectrometry has been widely used in many frontier fields such as proteomics,glycomics,metabolomics and disease diagnosis.Among them,drift time ion mobility(IMS)is the most mature ion mobility technology with the longest development time,it can obtain accurate collision cross section(CCS)values of ions,which provides information for analyzing ion structure and spatial conformation.On the other hand,high-field asymmetric wave ion mobility(FAIMS)is a special ion mobility technique,which uses the nonlinear change of ion mobility under high electric field to realize the selective separation of different kinds of ions based on the difference between high and low field ion mobility.Compared with foreign countries,domestic research on Ion mobility-mass spectrometry was not very comprehensive.In order to better develop the technology,this paper mainly carried out research from the following aspects:1.Based on previous research results,we systematically analysed the methods to improve FAIMS resolution;and SIMION software was used to build the planar FAIMS model,in which separation of different ions and compensation voltage scanning were simulated.2.Improve the original FAIMS platform.To achieve more stable and reliable detection results,the electrospray ion source power supply,electrospray needle,carrier gas generator,compensation voltage power supply,detection plate were replaced,and the structure of FAIMS device and the voltage waveform were optimized.3.Based on the new improved FAIMS platform,the parameters of electrospray voltage,curtain voltage and inject speed were optimized,the relationship between the separation voltage,ion type and FAIMS spectrum signal was verified,and the resolution of the existing platform was estimated using giving equation.4.The analysis of oligosaccharide isomers was carried out by drift time ion mobility-mass spectrometry.We systematically analyzed the separation of the IM spectra of oligosaccharide isomers,the fragmentation ions in MS-MS and the polymer phenomena in the IM-MS heat map.The average CCS value was calculated and corrected using single-field method,furthermore,a prediction method of CCS value was proposed. |
