Preparation And Its Action Mechanism Of Nano Basic Copper Sulfate Suspension Concentrate

Since the discovery of copper fungicide,it has occupied an important position in pesticide market with the characteristics of wide sterilization range,non-resistance,low toxicity and high efficiency.With the rapid development of a new technology nanotechnology,nanomaterials are becoming more and more popular.Their application in the field of pesticide can significantly improve the utilization rate of pesticides,reduce the amount of pesticide,and protect the environment,which is in line with the current social demand for green and environmentally friendly pesticides.At present,no nano-copper preparation is used in the market.Therefore,this dissertation choosed the nano basic copper sulfate to prepare suspension concentrate,through the indoor inhibition of bacteria,leaf inoculation,greenhouse pot test and field experiment to explore the mechanism and control effect of nanometer copper.The main results are as following:1.A 40% nano basis copper sulfate suspension concentrate(SC)has been developed successfully.In this dissertation,the additives and process conditions were screened,and the suspension rate and viscosity of the suspension samples were determined by grinding method.By comparing the properties of each sample,the formula of 40% nano basic copper sulfate SC with stable physical and chemical properties was obtained,that is,40% nano basic copper sulfate,20% 500#,1% SPSC3266,2% YUS-FS3000,1% SPSC2728,0.2% magnesium aluminum silicate A,3% xanthan gum,5% ethylene glycol and appropriate water,the material to medium ratio was 1:2.0 and grinded for 2 h.2.The antibacterial activity and its action mechanism of 40% nano basic copper sulfate SC were studied.The results showed that 40% basic copper sulfate SC could inhibit many kinds of pathogens more effectively than other copper fungicides,It can effectively inhibit tobacco pathogens such as Rhizoctonia solani,Alternaria alternate,and Pseudomonas syringae pv.tabaci through the Oxford cup inhibition test,the inhibition test of drug-containing medium,and the control test of in vitro leaves and pot culture.R.solani was selected as the research object,the morphology of mycelium,membrane permeability,lipid changes and DNA synthesis were studied,the results showed that 40% nano basic copper sulfate SC at the concentrations of 10 μg/ml,5 μg/ml and 2.5 μg/ml could break the hyphae of R.solani,cause lipid peroxidation and poor permeability of cell membrane,and block DNA synthesis.3.The effects of 40% nano basic copper sulfate SC on plant growth and physiological characteristics were studied.Compared with water control and 40% ordinary basic copper sulfate SC,spraying 40% nano basic copper sulfate SC can significantly increase the root length,plant height and dry matter accumulation of tobacco plants,and increase the chlorophyll content.At the same time,the copper content in root,stem and leaf increased significantly,and did not cause toxicity within a reasonable range.The activity of peroxidase(POD),polyphenol oxidase(PPO)and phenylalanine ammonia lyase(PAL)were significantly increased by 40% nano basic copper sulfate SC.40% nano basic copper sulfate SC also induced up regulation of NPR1 and PR1 genes related to plant disease resistance,Therefore,it is inferred that 40% nano basic copper sulfate SC can promote plant growth and induce resistance.4.The controling effects of 40% nano basic copper sulfate SC on some diseases in tobacco field were conducted.The results showed that the control effects of 40% nano basic copper sulfate SC on tobacco target spot,angular leaf spot and wildfire disease were better than the control,The control effect of 40% nano basic copper sulfate SC with diluted at 500 times on the three diseases was as high as 70%.This was basically consistent with the results of inhibition test in laboratory.There was no adverse effect on the growth and development of tobacco plants,and the yield and quality of flue-cured tobacco leaves were improved to a certain extent.In conclusion,40% nano basic copper sulfate SC is safe and effective for tobacco disease control and has broad application prospects.