Construction Of Three Polysaccharide-protein Nano-delivery Systems And Study On Their Loading Of Curcumin

As an ideal delivery vehicle,protein nanoparticles can be used for the delivery of lipophilic active substances and have a wide range of applications in the fields of food and medicine.However,protein nanocarriers are easy to agglomerate and unstable in the gastrointestinal tract.It is an effective method to improve the stability of protein nanocarriers by forming composite nanoparticles between proteins and polysaccharides.In this study,the transfer of functional active ingredient curcumin was taken as the research object,and the nanometer carrier based on protein and polysaccharide was studied to solve the problems of easy oxidation in vitro and low bioavailability of curcumin.The main contents were as follows:1.In this study,soybean protein isolate(Spi)and cellulose nanocrystals(CNC)composite nanoparticles were used as carriers for curcumin delivery(Cur).It is found that the mass ratio of Spi and CNC in the composite nanoparticles has a great influence on the stability and physical properties of Spi-CNC composite nanoparticles.When the mass ratio is 6:1,the particle size of Spi-CNC composite nanoparticles is smaller(197.7 nm),and the polydispersity index(PDI)is lower(0.14).At different pH values(3-9),temperature(30-90℃)and salt concentration(0-40 mmol/L),the Spi-CNC nanosystem was relatively stable.The encapsulation efficiency of composite nanoparticles reached 88.3%,and the composite nanoparticles could release curcumin continuously in simulated gastrointestinal digestion.2.A core-shell nanoparticle encapsulated and delivered curcumin was prepared by using soybean protein isolate(Spi)and fucoidan(FUC)as raw materials through electrostatic interaction under acidic and neutral conditions.The structures of Spi-Fuc nanoparticles were studied by Fourier transform infrared spectroscopy,transmission electron microscopy and scanning electron microscopy.The stability,drug loading rate and simulated release of Spi-FUC nanoparticles were also evaluated.The results showed that the prepared Spi-FUC nanoparticles(the particle size was about 236.56 nm)had a core-shell structure and could effectively load curcumin with an encapsulation efficiency of 95%.3.In this study,zein-AOS composite nanoparticles were prepared by antisolvent method based on alginate oligosaccharides(AOS)and zein,and their curcumin delivery performance was investigated.The results showed that when the weight ratio of Zein to AOS was 2:1,the particle size was 116.5 nm and the potential was-30 m V.Under the conditions of pH 4-9 and temperature 30℃-90℃,the composite nanoparticle system was relatively stable,and the encapsulation efficiency of curcumin reached 89.7%.The nanoparticle could reduce the release of curcumin in simulated gastric juice(SGF,pH 2.0)(lt;33%),the full release of curcumin in simulated intestinal fluid(SIF,pH 7.4),the release rate was 83.8%.