Metal-Organic Frameworks-based Fluorescence Sensors And Its Applications In Analysis

Fluorescence analysis means that the fluorescence properties of the analyte will be changed after the interaction between the fluorescent substance and the analyte,so as to realize the qualitative analysis and quantitative detection of the analyte.In recent years,fluorescence analysis has shown great application potential in the field of biological analysis because of its simple operation and high sensitivity.Metal-organic framework(MOFs),especially Fluorescent metal-organic frameworks(LMOFs),can effectively improve the detection sensitivity of fluorescence analysis due to their tunable pore size and size,excellent fluorescence performance,large specific surface area and good adsorption and other properties,but also a wide range of applications.Therefore,in this paper,three different fluorescence sensing platforms were constructed using LMOFs,and used for the detection of Cytochrome c(Cyt c)and the analysis of antibiotics in food and drugs.The main research content of this dissertation is divided into two parts as following:(1)As an electron transporter in the process of biological oxidation,Cyt c plays a very important role in cell apoptosis,so it is of great significance to detect it.In this part,we prepared ZAISQDs@MIL-68(In)complex by encapsulating Zn-Ag-In-S fluorescent quantum dots(ZAISQDs)in a Metal-Organic Framework(MIL-68(In))using a simple physical adsorption method,and used it for the detection of Cyt c.Under the optimal detection conditions,the linear concentration range of Cyt c was 0.02 μM to 3.5 μM,and the detection limit was as low as 8 nM.Finally,we successfully used it in the detection of Cyt c released by apoptotic cells and the screening of anti-tumor drug activity,which proved the practicability of ZAISQDs@MIL-68(In)complex in the detection of Cyt c and greatly enriched the application of MOFs in the field of life science.(2)As an antimicrobial drug,antibiotics are widely used in the world at present.However,excessive residues of antibiotics will affect people’s life,health and property safety.Therefore,in this chapter,we prepared three kinds of Eu3+and Tb3+co-doped MOFs-ratio sensor arrays for the identification and analysis of antibiotics.The results showed that when 25 different antibiotics were added,the three MOFs could produce different fluorescence responses,and the 25 antibiotics could be well identified at high(1 mM)and low(100 μM)concentrations in the presence of interfering substances.At the same time,the method has a high accuracy(98%)for the identification of 48 unknown antibiotic samples.Finally,the sensor array has been successfully applied to the analysis of antibiotics in pharmaceutical preparations and milk,which proves its practicability.