The Innate Targeting Ability Of PEI-PLGA Nanoparticle To Submandibular Salivary And Its Mechanism

Nanomedicines are defined as drug-containing nanoparticles with sizes ranging from 1 to 1000 nm.In recent years,nanomedicines have been developed rapidly,mainly including liposomes,polymers,inorganic nanoparticles,proteins,nucleic acids and other nanomaterials.There are also lots of nanomedicines approved for clinical usage for diseases treatment,such as doxorubicin liposome,albumin paclitaxel,etc.Nanomedicines can significantly prolong the half-life of free drugs in blood circulation and increase their aggregation in the disease site,thus achieving the effect of enhancing efficacy and reducing toxicity.Although a large number of nanomedicines have been developed,only a limited number of nanomedicines have been clinically approved.The main reason is that the structure of these nanomedicines is complex and is difficult to achieve large-scale production.At the same time,to active targeting ability of nanomedicine for disease sites,specific targeting groups need to be modified on the surface of nanoparticles,which further increases the complexity of nanomedicine and makes its clinical application more difficult.Therefore,the development of nanomedicines with simple structure,low cost and active targeting capability to disease sites has become a research hotspot.Studies showed that many nanoparticles with specific structure or specific ratio of different raw materials without any modification could target to specific organs or function with a specific pharmacological activity,such as liposome with different compositions ratio,can target to different organs,hyaluronic acid nanoparticles have nature targeting ability to CD44-positive cells,gold nanoparticles and silver nanoparticles have natural antiangiogenic activity,etc.This kind of medicine with natural targeting ability and drug activity can significantly overcome the problem of clinical transformation of complicated nanomedicines.As a cationic polymer,PEI has been widely used in the delivery of nucleic acid or protein drugs considering its interaction with many biomolecules.In these nanomedicines,the interaction between PEI polymer and drugs is mainly achieved by electrostatic interaction.The natural targeting properties of PEI to specific organs have not been reported so far.In this study,we found that PEI has a similar structural basis with acetylcholine receptor binding drugs.Therefore,we speculated that PEI-PLGA nanoparticles without any modification have the properties of binding to acetylcholine receptor.In vivo experiments showed that PEI-PLGA nanoparticles could effectively target submandibular salivary gland tissues.This work provides a nanomedicine with natural targeting properties to the submandibular salivary glands.At the same time,this strategy is also expected to provide a drug delivery system for salivary gland related diseases,which has a great potential for clinical application.