| China is a huge agricultural production country,and China’s agriculture is also accompanied by multiple and frequent diseases and insect pests which is extremely harmful to the growth of crops.However,in the past few decades,the unreasonable use and over reliance on chemical pesticides caused a large account of chemical residue and environmental pollution,which have seriously affected people’s lives and food security.Therefore,the concept of Green Prevention and Control which replaced chemical pesticides with biopesticides was put forward.After that,biopesticides are more and more accepted in agricultural production because of their specificity,no residue,no pollution and a serious of advantages such as infection and transmission.Nevertheless,there are also disadvantages such as single dosage form,unstable for large-scale mechanical operation,and over dependence on the environment,which lead to inactivate easily,and it’s unsuitable for long time storage and inability to long-term and slow release.In order to break through the limitation biopesticide,it is a potential research direction to optimize the dosage form and increase the availability factor of biopesticide.Organic-inorganic hybrid nanoflower is a kind of hybrid material with flower shape and self-assembled by organic ligands and metal ions,which applied to assemble biomacromolecules and improved their activity.Due to its high structural stability,densely distributed pore structure and large specific surface area improved the activity of enzyme molecules.Based on the above consideration,we replaced organic ligands with biopesticide in this paper and designed two kinds of biopesticide loading system with protein-inorganic hybrid nanoflower as substrates to improve the stability of biopesticides and achieve the effect of long-term slow release and long-term storage of biopesticides.The specific contents are as follows:Firstly,we used toxalbumin ‘Cry1Ac’ as organic-ligand to build Cry1Ac@Nanoflower system.Organic-inorganic hybrid nanoflower can not only load Cry1 Ac with a high efficient but also protect its activity for killing insects.Meanwhile,t it is able to self-disintegrate in insect intestinal and release Cry1 Ac later to kill pests.Above all,our system has an excellent ability to protect the protein structure and improve the stability of Cry1 Ac,which has solved the limitation that when Bt biopesticides were used in natural environment with a short action time and inactive easily.Secondly,we built Spore@Nanoflower system with organic-inorganic hybrid nanoflower and Beauveria bassiana spores.By the representation of our system,we proved that the spores were successfully loaded with a high efficient.Furthermore,our system can protect Beauveria bassiana spores and improve its germination activity after storing in room temperature.By evaluating the insect-resistant activity of our system,we proved that the system successfully protected spores and improve its insect-resistant activity.Therefor,this system proved that organic-inorganic nanoflower can load biopesticides and improved their insect-resistant activity.Moreover,our work laid the theoretical basis of assembly of other micro-organisms.In summary,our study proved that the biopesticides can be successfully assembled by orfanic-inorganic hybrid nanoflower,which can protect the stability and improve their insect-resistance activity,and achieve long-term release and long-term preservation of biopesticide insecticides.Therefore,our research provides a good idea to solve the limitations of biopesticides,and is of great significance to promote the application of biopesticides in green prevention and control. |
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