| Biothiol is an important part of cells in organisms,and it is an essential molecule for cells to play a major antioxidant role in cells.As the simplest thiol compounds,the related biochemical reactions and mechanism of H2S in cells are still the research focus of researchers.H2S can play an important role as a signaling molecule in the regulation of intravascular pressure and neural signal transmission in vivo.Related studies have shown that hydrogen sulfide can play an important role in promoting neuronal regulation,vasodilation,and apoptosis.However,the mechanism of hydrogen sulfide affecting intracellular signaling and regulating intracellular physiological processes is still in the research stage.Due to the important role of H2S in the process of interaction and unique biochemical reactions,it is of great significance to study its production,function and metabolism in biological tissues.Therefore,it is necessary to study the detection method of hydrogen sulfide with excellent performance.The fluorescent probe has a good selectivity for the substance and a good detection effect for the trace substance.It has become an important detection method in the field of biological imaging due to its less damage when it is used for cell detection.In this thesis,a specific probe sensitive to hydrogen sulfide was designed and synthesized.The main contents are as follows:A ratio fluorescent probe CDs-PNBD for H2S detection was synthesized in a simple and easy way.The probe CDs-PNBD consists of two parts:one is the NBD-based derivative P-NBD which is sensitive to hydrogen sulfide and has yellow fluorescence.The other part is doped carbon quantum dots with blue fluorescence.Under the action of hydrogen sulfide,the P-NBD part undergoes thiolysis,and the C-N bond is broken to form red NBD-SH.The yellow fluorescence of P-NBD was quenched.Because the fluorescence emission spectrum of carbon dots overlaps with the ultraviolet absorption spectrum of P-NBD,the blue fluorescence of carbon dots is partially quenched due to the fluorescence resonance energy transfer effect.Under the action of hydrogen sulfide,the FRET effect is weakened,and the blue fluorescence of carbon dots is enhanced.Therefore,with the increase of H2S concentration,the fluorescence of the probe CDs-PNBD changes from yellow to blue under the ultraviolet lamp.When interacting with other related molecules,the fluorescence of the probe was basically unchanged,and the probe showed good selectivity for H2S.The detection limit of the probe molecule for hydrogen sulfide reaches 57 nM,which exceeds most of the conventional detection methods,and can be used for the detection of trace H2S.Finally,the probe was successfully used to detect H2S in real water samples. |
