Preparation Of BSA-tannic Acid System And Its Application In Delivering Caffeic Acid Phenethyl Ester

Serum albumin is the most abundant protein in human and animal blood.Due to its high solubility and good stability,they are very important in various life activities in human and animal body.effect.Caffeic acid β-phenylethyl ester(Caffeic acid β-phenylethyl ester,CAPE)is an important component of caffeic acid derivatives and propolis activity,and has better anticancer activity than caffeic acid.There are few reports on the research of CAPE transportation system and operation behavior,so it is of great significance to carry out the construction and application of CAPE system based on serum protein transportation.This article is devoted to exploring human serum albumin(HSA)and bovine serum albumin(BSA)as carrier proteins,and CAPE as ligand molecule,through multispectral techniques to study the interaction between protein and CAPE,to clarify HSA / BSA-CAPE complex formation mechanism;clarify the protective effect of complex formation on protein and polyphenols through thermal stability analysis,UV stability and high temperature stability;adopt layer-by-layer assembly method to construct BSA-tannic acid carrier And study its application in carrying BSA-CAPE.The results showed that CAPE caused static quenching of both HSA / BSA,indicating that the binding reaction between HSA / BSA and CAPE formed the HSA / BSA-CAPE complex.The HSA affinity and binding sites were significantly higher than that of BSA;Stability of protein and polyphenols;using food-grade polylysine instead of macromolecular polylysine to construct the delivery system,successfully prepared microencapsulated CAPE,the embedding rate of BSA-CAPE reached 59.24 ± 1.16%,The loading capacity of CAPE is 68 ± 0.62 mg / g.The in vitro simulated digestion experiment proves that microencapsulated CAPE is effective against gastric digestion,has a slow-release effect in intestinal digestion,and effectively improves the CAPE bioavailability.