| Streptomyces coelicolor A3(2)is a model strain of Streptomyces.It can produce many kinds of antibiotics and other medicinal compounds,and its complex mechanism of morphological differentiation and secondary metabolic regulation has always been a research hotspot.However,as the main strain in the industrial production of antibiotics,many aerial mycelia are formed during the production process,which results in the high density of the mycelium,the over consumption of nutrients and oxygen,and the decline of antibiotic production,seriously affecting industrial production efficiency.SCO4114,a sporulation-related protein,was annotated on the NCBI website and its function is unknown.In our previous study,we constructed △sco4114 strain.Phenotypic analysis showed that the growth of aerial mycelium was blocked.Bioinformatics analysis showed that sco4114 was the target gene of sporulation-related s RNA scr4115.On the basis of previous studies,we further explored the function of sporulationrelated protein SCO4114.The relationship between s RNA scr4115 and its target gene sco4114 was studied in S.coelicolor.It was found that sco4114 was a prominent activating target of scr4115 and shared a bidirectional promoter.The effects of SCO4114 on sporulation were quantitatively analyzed by i TRAQ and LC-MS/MS at the protein level.Compared with WT strains,there were 77 up-regulated proteins and 86 down-regulated proteins in △sco4114.These distinct differential proteins are involved in carbon metabolism,ABC transport system,signal transduction,ribosomal protein and other metabolic and regulatory pathways.The analysis of these differential proteins indicates that SCO4114 may be involved in the regulation of bld family genes,thus affecting the formation of aerial hyphae.At the same time,GST-SCO4114 fusion proteins were constructed in E.coli and 79 proteins interacting with SCO4114 were identified by pull down technique.It was further found that SCO4114 protein may directly affect the expression of bld KB gene,thus affecting the morphological differentiation of S.coelicolor.To sum up,we made a preliminary analysis of the functions of SCO4114 through the above experiments,and found a number of proteins that may interact with it,expanding the regulatory mechanism of the morphological differentiation of S.coelicolor,which is of great significance for further improving the morphological differentiation network of S.coelicolor. |
