Effect Of Shirebijiaonang On Rheumatoid Arthritis And Its Metabolism Studies In Rats

Object:To investigate the mechanism of Shirebijiaonang(SRBJN)on treating rheumatoid arthritis.An Ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS)method was used to quantify the chemical constituents and metabolites of SRBJN.Providing a material basis of the potential active chemical constituents for SRBJN.Methods:1.The pharmacodynamic effects of SRBJN on the treatment of acute inflammation were investigated through xylene-induced ear edema model in mice.The pharmacodynamic effects of SRBJN on relieving inflammatory pain were investigated by writhing model induced by acetic acid in mice.The effect of SRBJN on paw swelling was investigated by rat rheumatoid arthritis model induced by complete Freund’s adjuvant(CFA).2.The chemical composition of SRBJN and the metabolites in the rat’s plasma,feces and urine after administrated of SRBJN was analyzed by ultra-high performance liquid chromatography-tandem mass spectrometry.Results:1.SRBJN can significantly inhibit xylene-induced mice ear edema;attenuate acetic acid-induced pain;attenuate the rat paw swelling,and maintain spleen and thymus index in CFA-induced rat arthritis model.Additionally,decline of inflammatory factors interleukin-6(IL-6),interleukin-1beta(IL-1β)and tumor necrosis factor-alpha(TNF-α)in the serum were observed in the SRBJN-treated rats.2.Totally 108 compounds were identified in SRBJN by ultra-high performance liquid chromatography-tandem mass spectrometry;7 prototype drug components were identified in plasma samples;6 prototype drug components were identified in urine samples;11 prototype drug components were identified in the fecal samples.Conclusions:1.SRBJN has obvious anti-inflammatory and analgesic effects,and preventive and therapeutic effects on rheumatoid arthritis.The mechanism may be related to reducing inflammation and improving immunity.2.The chemical composition of SRBJN was clarified,7 prototype drug components including succinic acid,syringin,and glucosides etc.were identified in plasma samples;6prototype drug components including Loganic acid,loganin and Fangchinoline etc.were identified in urine samples.Eleven kinds of drug prototype components including atractyoldinol,5-hydroxymethylfurfural,and chlorogenic acid were identified in fecal samples.