Design,Synthesis Of Naphthalimide Fluorescent Probes And Its Application On Active Small Molecule Detection

Endogenous active small molecule such as glutathione and superoxide radical are closely related with a variety of life activities of cells and tissues.They are important substances which are involved in the body’s immune,material and energy transportation,signal transmission and the maintenance of internal stability.At the same time,the content of these substances is related to the pathological study of many kinds of diseases,including cancer,inflammation and various kinds of nervous diseases,which are accompanied by the abnormal secretion of these substances.Therefore,With The advantages of the 1,8-Naphthalimide such as double photon excitation and high fluorescence intensity,which the traditional fluorescent materials don`t have to design highly sensitive fluorescent probes for these substances is of great significance.This topic is carried out on the following aspects:1.Study the naphthalimide based π-π quenching performance,by changing the parameters of factors influencing the fluorescence quenching efficiency.Establish the dual-naphthalimide self-quenching model,take a comprehensive study on the performance of fluorescence of naphthalimide monomer and two polymer,to provide theoretical basis for the application of the probe.2.Apply the π-π stacking self-quenching characteristic of naphthalimide for the design and synthesis of glutathione probe,to get the GSH fluorescent probe with dual channel and two photon properties.The results show that the probes are effective and sensitive in the detection of endogenous glutathione.On the other hand,the cell permeability of the probe is good,the signal intensity is high,and it can be used in two photon imaging experiments.3.Based on the principle of intramolecular charge transfer(ICT),design and synthesize a probe for detecting the superoxide radical using the naphthalimide.The results show that the probe has good detection performance,high specificity and stability,and it has the advantage of fast expression.In summary,the detection performance of the probe is good,and the specificity is strong,which can be used as a new fluorescent probe for detection of the target substance.