Electrochemical Hydrogen Peroxide Sensor Based On PtCu Alloy Composite Prepared In Deep Eutectic Solvents And The Interaction Between Several Copper Complexes And DNA

Studying the interaction of metal complex with DNA and exploring their interaction mechanism,are of great importance for the preparation of anticancer drugs with little side effect,good curative effect and in vitro screening of anticancer drugs,and the exploration of the anticancer drugs’nature in vivo treatment.In this thesis,the interaction between the ligand(C₁₄H₁₅NO₂)and its two Cu complexes with DNA was studied by electrochemical method and UV-Vis spectroscopy.In addition,with the deep eutectic solvents（DESs）as the medium,the PtCu alloy composite was deposited on a glassy carbon electrode by electrochemical method and a new non-enzyme electrochemical H₂O₂ sensor was successfully fabricated.The main original results obtained are as follows:（1）The interaction between the ligand(C₁₄H₁₅NO₂)and its two copper complexes[Cu₂₀(C₁₄H₁₅NO₂)₂₀ and Cu₈(C₁₄H₁₅NO₂)₄（OH）₄（H₂O）₄（ClO₄）₂]with calf thymus DNA was investigated by using ultraviolet visible（UV-vis）absorption spectroscopy and electrochemical methods.Their interaction conditions were optimized,and the interaction mode was explored.The results show that ① the ligand(C₁₄H₁₅NO₂)binded with DNA mainly through electrostatic interaction,and accompanied by a weak insertion.Moreover,the binding ratio（m）and the equilibrium constant（β）of the binding reaction between ligand(C₁₄H₁₅NO₂)and DNA were calculated to be 2 and 4.32,respectively;②the Cu₂₀(C₁₄H₁₅NO₂)₂₀ complex binded with DNA mainly through electrostatic interaction,and accompanied by a weak insertion.Moreover,the binding ratio（m）and the equilibrium constant（β）of the binding reaction between Cu₂₀(C₁₄H₁₅NO₂)₂₀ complex and DNA were calculated to be 1 and 2.12×10³,respectively.③ the Cu₈（Ci₄Hi₅NO₂）₄（OH）₄（H₂O）₄（ClO₄）₂ complex binded with DNA mainly through electrostatic interaction,and accompanied by a weak insertion.Moreover,the binding ratio（m）and the equilibrium constant（β）of the binding reaction between Cu₈(C₁₄H₁₅NO₂)₄（OH）₄（H₂O）₄（ClO₄）₂ complex and DNA were calculated to be 2 and 8.93×10⁶,respectively.（2）With the choline chloride/ethylene glycol DESs as the medium,the PtCu alloy composite was deposited on a glassy carbon electrode by electrochemical method and a new non-enzyme electrochemical H₂O₂ sensor was fabricated.The response properties of this sensor to H₂O₂ were investigated by cyclic voltammetry and chronoamperometry,and its preparation conditions were optimized.Under the best conditions,the sensor for H₂O₂ detection has a wide linear range,low detection limit,high sensitivity and good stability（the amperometric response can still maintain 92.7%of its initial response current after 7200 s）,as well as excellent reproducibility（four parallel test,relative standard deviation is 4.2%）and anti-disturbance performance（the addition of L-tyrosine,L-arginine（L-Arg）,glucose,ascorbic acid and dopamine have no significant effect on the H₂O₂ detection）.