Fabrication And Properties Of SF/PLCL/Gelma Electrospinning Barrier Membrane

Objective: To prepare SF/PLCL/Gelma fiber barrier membranes by blending electrospinning technology,and to compare the surface morphology,internal structure,hydrophilic,mechanical property,degradation property,cytotoxicity and osteogenic properties of barrier membranes with different Gelma contents,so as to provide preliminary data for application in the field of guided bone regeneration membranes.Methods: Depending blending electrospinning technology,using hexafluoroisopropanol as solvent and SF/PLCL/Gelma with a mass ratio of 3:7:X(X=0,2,4,6)as solute.,the mass volume fraction of SF/PLCL was 8%,the voltage of 11 Kv was loaded,the needle-to-pole distance was 12 cm,the advancing speed was0.02ml/min,the temperature was 30?-35?,and the humidity was 25%-30%.Four different fiber membranes of G0,G2,G4 and G6 were obtained by electrospinning.Then,the surface morphology of the fiber membrane was observed by scanning electron microscope,the functional groups and crystal conformation of the fiber membrane were detected by Fourier transform infrared spectroscopy and X-ray diffraction,the hydrophilicity of the sample were tested by the contact angle experiment,and the mechanical property of the sample was tested by a tensile testing machine.Degradation experiments were carried out to detect the degradation properties of fiber membranes.The rabbit bone marrow mesenchymal stem cells(r BMSCs)were extracted by the whole bone marrow adherent method,and then the adhesion of r BMSCs on the fibrous membrane was detected by FDA staining assay,and the proliferation of r BMSCs on the fibrous membrane was detected by CCK-8 assay.The effect of fibrous membranes on the osteogenic differentiation of r BMSCs was determined by quantitative assay of alkaline phosphatase.Results: 1.Scanning electron microscope results showed that the surface of the fibrous membrane in each group was smooth,continuous,uniform in thickness,without obvious bead-like defects,and the fibers in each group were disorderly arranged in a three-dimensional network.There were significant statistical differences in fiber diameter between G2,G4,G6 groups and G0 group.2.Fourier transform infrared spectroscopy results show that the four groups of fibrous membranes have characteristic peak of SF and PLCL,and G2,G4,G6 groups have characteristic peak of Gelma,indicating that Gelma is successfully electrospun into the fibrous membrane and has no denaturation.3.X-ray diffraction results showed that the diffraction peaks of SF and PLCL were expressed in the four groups of fibrous membranes.The introduction of Gelma material may cause the diffraction peaks of silk fibroin to be slightly left-shifted(reduced).4.The results of the contact angle test showed that the fibrous membranes in each group were hydrophilic membranes.The introduction of Gelma material reduced the hydrophilicity of the fibrous membrane,but the degree of reduction was not significant.The degree of reduction of the fibrous membrane in the G2 group was minimum.5.The tensile test results showed that the introduction of Gelma material significantly increased the tensile strength of the fibrous membranes,among which the tensile strength of the fibrous membranes in the G2 group was the largest;it also increaseed the elastic modulus of the barrier membranes,but the difference was not statistically significant.In addition,the elongation at break of the material was reduced,but only the G6 group was statistically different from the control group.6.The results of the degradation experiments showed that the barrier membranes of each group could be degraded.During the first 9 weeks,the degradation of the barrier membranes in the G0 group gradually accelerated,and the degradation of the barrier membranes in the G2,G4,and G6 groups gradually slowed down.7.FDA staining results showed that r BMSCs could adhere well and maintain a certain activity on the fibrous membranes of each group.8.The results of CCK-8 experiment showed that the fibrous membranes of each group had no cytotoxicity to r BMSCs,the latter could proliferate well,and the cell proliferation rate of G2 group was the fastest.9.The results of ALP quantitative experiments showed that compared with the control group,the ALP activity of the fibrous membranes in each group was slightly increased but did not significantly promote the osteogenic differentiation of r BMSCs.Conclusion: The SF/PLCL/Gelma fibrous barrier membranes was successfully prepared by electrospinning technology.The G2 group with solute mass ratio of MSF:MPLCL:MGelma=3:7:2 had better hydrophilic properties,excellent mechanical properties,and degradation time more in line with the clinical requirements,great biocompatibility,and possessing a certain ability to induce osteogenesis.It has the potential for clinical application in implantation.However,its pro-osteodifferentiation ability needsed to be further verified.