Preparation Of Polyacrylate Resin By Seed Emulsion Polymerization And Application In Separation And Enrichment

As a kind of common molecular polymeric material,synthetic resin exhibits good physical and chemical stability and has widely applied in the fields of polymer adsorption,separation and catalysis.In order to fulfill the requirement of biological samples analysis,the development of novel functionalized resins is still one of the research hotspots in the field of materials.Herein,two kinds of novel MARs were successfully designed and fabricated as following.First,a composite resin material with core-shell structure was prepared with polyacrylate resin microspheres as the core and nanodiamond as the shell.Specifically,the amino-modified nanodiamond particles were integrated onto the surface of micron-sized microspheres to prepare composite material with amino groups on the surface.Then the amino groups on the microspheres underwent aminoaldehyde condensation reaction with2,3,4-trihydroxybenzaldehyde to introduce pyrogallol groups.Finally,phenolic hydroxyl groups were chelated with titanium ions(Ti⁴⁺)to prepare composite adsorbents for immobilized metal affinity chromatography（IMAC）.The prepared composite resin possessed excellent specificity and selectivity for the phosphopeptides in the standard proteolysis solution,and 106 phosphopeptides from 29 phosphorylated proteins could be identified from 5μL of milk proteolysis.Taking advantage of stronger chelation interaction between Ti⁴⁺and pyrogallol group in 2,3,4-trihydroxybenzaldehyde than that between Ti⁴⁺and phosphate group in phosphopeptide,Ti⁴⁺would not be dissociated from the composite resin after a whole enrichment process.Therefore,the IMAC adsorbent could be utilized repeatedly.The results showed that it still maintains stable enrichment performance after repeated use for 4 times.The core-shell composite resin material prepared in this study has great application potential in phosphoproteomics and is expected to be commercialized.Anisotropic particles（also known as Janus particles）are a new type of asymmetric material,exhibiting a variety of special properties and functions compared with uniform or symmetric materials and gradually attracting widespread attention.In this chapter,three kinds of Janus particles with different morphologies were prepared by seed emulsion polymerization.Specifically,glycidyl methacrylate（GMA）was selected as functional monomer,and dipentaerythritol penta-/hex-acrylate（DPEPA）containing multiple acrylate functional groups as cross-linking reagent.Janus particles with half raspberry,dumbell and egg shape were prepared by UV light-initiated seed emulsion polymerization.The modification of Janus particles are undergone by atom transfer radical polymerization（ATRP）and amination modification.It is expected these Janus particles to be applied in the adsorption of heavy metal ions.