SERS Study Of MOF-based Nano-enzyme Material And Its Application In Antibacterial Field

Surface-enhanced Raman scattering（SERS）is a non-damaging trace analysis and detection technology that can provide the inherent structure information of molecular or nanomaterial surfaces.Due to the high sensitivity of SERS technology,even the detection level at the single molecule level,SERS technology has attracted wide attention and great application in many fields,especially the detection of pathogenic bacteria and the application of antibacterial in the biological field.Metal-organic framework（MOF）and MOF matrix composite materials can be used as very good SERS substrates,and their SERS enhanced effect is not less than that of traditional SERS substrates.Moreover,due to the permanent pores,large specific surface area and active sites,MOF has greater advantages in stability and selectivity.And MOF matrix composites used in addition to can serve as a good SERS substrate,because of the load with a large number of active site,causes it to be more strong class peroxidase enzyme catalytic oxidation performance,and of multiple drug resistance bacteria showed great resistance,leading to MOF nano-enzyme composite material in the biological nanometer medicine caused a very extensive research.In this paper,MOF-based nano-enzyme composite material with enzyme-like catalytic performance,SERS activity and antibacterial properties was used as SERS substrate in the field of antibacterial.The research contents are as follows:1)Based on the SERS activity of two-dimensional porphyrin MOF[Cu-Tcpp(Fe³⁺)]and peroxidase-like catalytic activity,a nano-enzyme-catalyzed SERS detection system was constructed,and the SERS real-time monitoring of two-dimensional porphyrin MOF nanosheet surface catalytic reaction was conducted.It not only realized the application of enzyme-like catalytic activity of nano-enzyme materials in SERS field,but also provided a new detection platform for in-situ real-time monitoring of catalytic reactions.2)Two-dimensional MOF-based nano-enzyme composite Au@Cu-Tcpp(Fe³⁺)was prepared by using two-dimensional porphyrin MOF nanosheets as a carrier loaded with gold nanoparticles.The two-dimensional MOF-based nano-enzyme composite not only retained the strong enzyme-like catalytic performance of two-dimensional porphyrin MOF,but also due to the composite of gold nanoparticles.The SERS activity and peroxidase-like catalytic performance of the nanoenzyme composites were also significantly improved.Using the enzyme-like catalytic performance and SERS activity of two-dimensional MOF-based nano-enzymematerial Au@Cu-Tcpp(Fe³⁺)as the architecture,the nano-enzyme catalyzation-SERS detection platform was established.Both the peroxide-like catalytic performance and SERS activity were further improved.This super-active in-situ monitoring platform was applied to the field of antibacterial,and the SERS detection platform catalyzed by nano-enzyme was further constructed.The killing of pathogenic bacteria Staphylococcus aureus was realized with the help of the enzyme-like catalytic oxidation performance of the material.At the same time,SERS technology was used to monitor the antibacterial process,which realized the antibacterial detection and analysis on the single molecule level in the real sense.Finally,this paper designed a nano-enzyme surface-enhanced Raman antibacterial detection kit,which truly applied the SERS monitoring detection mechanism of nano-enzyme-catalyzed antibacterial to practical application,and had a very wide application potential in the biomedical field.