| Natural enzymes can effectively catalyze a variety of biochemical reactions in the body of organisms,but the instability and high cost of natural enzymes and other shortcomings limit their application in actual production and life.Recently,some nanomaterials have been found to have the activity of natural enzymes.Such nanomaterials with the activity of natural enzymes are called nanomaterials.Among many kinds of nanozymes,nanozymes that have peroxidase-like activity have been widely studied.In this paper,g-C3N4-based nanomaterials were prepared,their peroxidase-like activity was studied and their applications in the detection of H2O2 and glucose were explored.By using melamine and cyanuric acid as precursors,iron-doped carbon nitride two-dimensional ultrathin nanosheets（Fe-g-C3N4 NSs）were prepared by supramolecular self-assembly method.It was found that Fe-g-C3N4 NSs had peroxidase-like activity.The catalytic kinetics of Fe-g-C3N4 NSs showed that its affinity for H2O2 and 3,3,5,5-tetramethylbenzidine（TMB）was higher than that of natural horseradish peroxidase（HRP）.Through the study of the catalytic reaction mechanism of Fe-g-C3N4 NSs,it was found that the peroxidase activity of Fe-g-C3N4 NSs came from hydroxyl radical（·OH）.Based on the peroxidase activity of Fe-g-C3N4 NSs,it was successfully applied in the detection of H2O2in milk with the standard recovery of 97.22-102.64%and the relative standard deviation（RSD）lower than 3.04%.Therefore,this method has high accuracy and good practical application prospect.At the same time,the detection of glucose in human blood samples by Fe-g-C3N4 NSs was successfully realized.The standard recovery was 100.56-107.39%,and the RSD was lower than 5.04%.It was proved that Fe-g-C3N4 NSs had good selectivity for the detection of glucose in human blood samples.Based on Fe-g-C3N4 NSs,Fe-g-C3N4 NSs and Mo S2 were further combined to synthesize iron-doped carbon nitride molybdenum disulfide composite with intercalation structure（Mo S2/Fe-g-C3N4 NSs）.It was found that Mo S2 Fe-g-C3N4NSs had peroxidase activity.The catalytic reaction mechanism of Mo S2/Fe-g-C3N4 NSs was studied.It was found that the peroxidase activity of Mo S2/Fe-g-C3N4 NSs came from the introduction of Mo S2 and it can accelerates the transfer of electrons.Meanwhile,Mo S2/Fe-g-C3N4 NSs promoted the conversion of H2O2to·OH.Mo S2/Fe-g-C3N4 NSs was successfully applied to the detection of H2O2in milk,with the spiked-recovery rate of 97.15-100.02%and the RSD of 0.53-2.14%.At the same time,Mo S2/Fe-g-C3N4 NSs was successfully applied in the detection of serum glucose,and the spiked-recovery rate was in the range of102.30-103.43%,and the RSDs were all less than 6.62%,indicating that it was feasible to apply Mo S2/Fe-g-C3N4 NSs in the detection of serum glucose. |
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