Research On The Construction And Properties Of Phototheranostic Nanoparticle Based On BODIPY And Graphene

In view of the limitations in the current cancer treatment,theranostic provides a new vision on cancer treatment.BODIPY is an excellent fluorescent dye and is being researched in the field of cancer phototherapy.But there are few reports that the prepared BODIPY nanomaterials have PDT,PTT and FLI effects,and there is still lack of research.In order to prepare BODIPY nanoparticles with PDT,PTT and FLI effects,we acquired nanoparticle MGBP by inducing amphoteric compound BODIPY-PEG(BP)self-assembled in the surface of two-dimensional material graphene.MGBP nanoparticles possess the therapeutic effect of chemotherapy,PDT and PTT,and the potential of fluorescence imaging and photothermal imaging.(1)To improve the water solubility of BODIPY,the derivative BP were prepared.In this paper,the drug-loaded graphene MMC-graphene(MG)and BP are used for preparing MGBP.BP self-assembled on the surface of MG under the action of ?-? stacking and hydrophobic bonds to form MGBP nanoparticles.The existence of BP can improve the water solubility and stability of MGBP,BP can also inhibit the drug release in MG so as to achieve the effect of drug sustained release.BP is the photosensitizer of PDT,giving MGBP the PDT effect.MG gives MGBP the effects of PTT and chemotherapy.The structure of MGBP nanoparticles was confirmed by TEM and UVVis,and the stability of MGBP nanoparticles was confirmed by Zeta potential and particle size analysis.(2)The various properties of MGBP nanoparticles are researched: Fluorescence imaging and photothermal imaging effects,ROS generation effects,photothermal conversion efficiency,photothermal stability,the influence of various factors on photothermal conversion efficiency,and two therapies by another excites light.Finally,the following conclusions were confirmed: MGBP can effectively perform fluorescence imaging and photothermal imaging in the agar model,and can effectively generate ROS.The photothermal conversion efficiency is 48 %.After four cycles of heating-colding processes,MGBP still maintains the original efficiency.The 808 nm laser has PTT effect but no PDT effect,and the 505 nm laser has both PTT and PDT effect.(3)The cellular uptake of MGBP nanoparticles and the therapeutic effect of nanoparticles on tumor cells are researched.Through laser confocal experiment and flow cytometry,it was found that MGBP nanoparticles can be taken up by cells,and the uptake mode was different from that of molecular BP.The MTT method was used to study the treatment of tumor cells by PDT,PTT and chemotherapy.It was found that after synergistic treatment with PTT & PDT & chemotherapy,the cell survival rate dropped to 17 %,showing good treatment effect.