Preparation Of Copper/Silver Nanoclusters And Its Application In Glioma And Novel Object Recognition Memory

Glioblastoma multiforme(GBM)is the most common and aggressive primary brain tumor.For a long time,people have made great efforts to develop a nano-drug carrier system that can cross the blood-brain barrier(BBB).In this paper,bovine serum albumin-silver nanoclusters with excellent biocompatibility was designed.Based on the electrostatic interaction with paclitaxel(PTX),they were further formulated into spherical BSA-AgNc-paclitaxel nanoparticles(AgNc-PTX-NPs).BSA-AgNc-NPs system can efficiently pass through the BBB and deliver the drug to the tumor site,whether in vitro or in vivo.In vivo assessment of AgNc-PTX-NPs on glioblastoma multiforme(GBM)bearing mice revealed significant inhibition of tumor growth and migration rendering prolonged survival of the mice.photothermal therapy and immunotherapy to kill tumor cells while reducing the risk of local recurrence through limited injection times.If there is a material that can selectively remove a specific short-term memory(such as a car accident,murder,etc.),and subsequently excrete it automatically from the body,it will be expected to reduce depression,anxiety,and even suicidal events significantly.The bovine serum albumin(BSA)-templated copper nanoclusters(BSA-CuNCs)and silver nanoclusters(BSA-AgNCs)was synthesized respectively in this paper,and we discuss their potential as short-term memory “erasers”.Due to the small size of metal nanoclusters(1-4 nm),both copper or silver nanoclusters can efficiently penetrate the BBB and decrease the novel object recognition memory of mice within 24 hours of entry into brain.Interestingly,after 3 weeks,the discrimination index(DI)of the BSA-CuNCs-treated mice increases and the memory can be recovered.In contrast,mice of the BSA-AgNCs group do not regain their memory,and similar results are obtained with further high-dose injection of BSA-CuNCs.In addition,BSA-CuNCs exhibit excellent biocompatibility and can be metabolically cleared by the organism,which make BSA-CuNCs promising candidates for short memory erasure.