Study On The Detection Of Antibiotic Residues In Milk Based On Three-dimensional Fluorescence Spectroscopy

In recent years,the serious harm caused by excessive antibiotic residues has gradually attracted people's attention.Improper discharge of animal husbandry wastewater and medical wastewater in daily life causes the accumulation of antibiotics in the soil and water bodies,resulting irreversible damage to the ecological environment.At the same time,the abuse of antibiotics in the process of animal husbandry has left excessive amounts of antibiotics in animal-derived food,which seriously endangers human health.This paper combined three-dimensional fluorescence spectroscopy technology and second-order correction algorithm to detect several common antibiotics in water and milk,which is of great significance for ensuring food safety and environment protection.The main research work are as follows:(1)The structures and characteristics of three quinolone antibiotics,flumequine(FLU),enrofloxacin(ENR)and ciprofloxacin(CIP)were studied to determine the feasibility of three-dimensional fluorescence spectroscopy detection.FS920 steady-state fluorescence spectrometer was used to detect the three antibiotic samples.The collected spectral data were preprocessed to analyze the fluorescence characteristics of three antibiotics.(2)Three denoising algorithms: Ensemble Empirical Mode Decomposition(EEMD)Wavelet optimized EEMD and Complementary Ensemble Empirical Mode Decomposition(CEEMD)were used to denoise the spectral data.By comparing evaluation indicators,CEEMD was determined as the optimal denoising algorithm for subsequent data processing.(3)Three groups of pure material experimental samples with different components were designed to study quinolone antibiotics in water.Two second-order calibration algorithms(Bilinear Least Squares with Residual Bilinearization and Unfold Partial Least Squares)were used for qualitative and quantitative detection of experimental samples.By comparing various parameters of the experimental results,it was demonstrated that the UPLS/RBL algorithm was more suitable for detecting quinolone antibiotics in water.(4)In response to the problem of quinolone antibiotic residues in milk,three sets of milk spiked antibiotic residue experiments were designed.The milk was subjected to protein precipitation,and aluminum ion solution was used to enhance the fluorescence intensity of antibiotics in the spiked samples.The experimental results further demonstrated that threedimensional fluorescence spectroscopy technology combined with U-PLS/RBL algorithm can still accurately qualitatively and quantitatively detect the mixed components of quinolone antibiotics under the interference of the complex background of milk.